Cartilage synthesizes the serine protease inhibitor PAI-1: Support for the involvement of serine proteases in cartilage remodeling
| Autor: | Henry J. Mankin, Christine A. Towle, Michele Pavia, Vernon J. Cooley, Benjamin V. Treadwell |
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| Rok vydání: | 1991 |
| Předmět: |
Electrophoresis
Serine protease Proteases Cartilage Plasminogen Biology Blotting Northern Precipitin Tests Tissue plasminogen activator Molecular biology Chondrocyte Serine Plasminogen Inactivators medicine.anatomical_structure Tissue Plasminogen Activator medicine biology.protein Humans Orthopedics and Sports Medicine RNA Messenger Northern blot Plasminogen activator Interleukin-1 medicine.drug |
| Zdroj: | Journal of Orthopaedic Research. 9:309-316 |
| ISSN: | 1554-527X 0736-0266 |
| DOI: | 10.1002/jor.1100090302 |
| Popis: | The work described here demonstrates the synthesis by human articular cartilage of plasminogen activator inhibitor-1 (PAI-1), a potent inhibitor of the serine protease tissue plasminogen activator (tPA). We also present data demonstrating an increase in PAI-1 messenger ribonucleic acid (mRNA) in chondrocytes exposed to the cytokine interleukin-1 (IL-1). Interestingly, this elevation of steady-state mRNA levels does not appear to result in an increase in synthesis of PAI-1 protein. Northern blot analysis reveals that of the two mRNA species (3.4 kb, 2.4 kb) previously reported for PAI-1, only the larger species (3.4 kb) appears to be synthesized by chrondrocytes. Our data demonstrate the IL-1-stimulated production by cartilage of tissue plasminogen activator. We also show evidence for the presence of plasminogen in cartilage. A scheme is presented indicating the probable importance of the serine proteases (tPA and plasminogen) and PAI-1 in cartilage degradation. |
| Databáze: | OpenAIRE |
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