An alternative model of H ferritin promoter transactivation by c-Jun
| Autor: | Faniello, Maria C, Chirico, Giuseppa, Quaresima, Barbara, Cuda, Giovanni, Allevato, Giovanna, Bevilacqua, Maria A, Baudi, Francesco, Colantuoni, Vittorio, Cimino, Filiberto, Venuta, Salvatore, Avvedimento, Vittorio E, Costanzo, Francesco |
|---|---|
| Přispěvatelé: | Faniello, Mc, Chirico, G, Quaresima, B, Cuda, G, Allevato, G, Bevilacqua, MARIA ASSUNTA, Baudi, F, Colantuoni, V, Cimino, F, Venuta, S, Avvedimento, Ve, Costanzo, F. |
| Rok vydání: | 2002 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Transcriptional Activation Binding Sites Transcription Genetic Proto-Oncogene Proteins c-jun Recombinant Fusion Proteins Blotting Western Nuclear Proteins Cell Biology Transfection Precipitin Tests Biochemistry Recombinant Proteins Cell Line Protein Structure Tertiary Ferritins Cyclic AMP Trans-Activators Humans Promoter Regions Genetic Molecular Biology Research Article HeLa Cells Protein Binding |
| Zdroj: | Biochemical Journal. 363:53-58 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3630053 |
| Popis: | c-Jun is a member of the activator protein 1 family, and its interaction with different nuclear factors generates a wide spectrum of complexes that regulate transcription of different promoters. H ferritin promoter transcription is tightly dependent on nuclear factor Y (NFY). Ferritin transcription is activated by c-Jun, although the promoter does not contain a canonical binding site. NFY, on the other hand, does not bind c-Jun in vitro, whereas in vivo c-Jun is found in the complex containing NFY. Moreover, a c-Jun—GCN4 chimaeric construct containing only the transactivation domain of Jun and the basic-region leucine-zipper domain of GCN4 stimulates the H ferritin promoter. A synthetic GAL4 promoter and the cognate activator, the fusion protein NFY—GAL4, are potently activated by c-Jun. Titration of p300 by co-expressing E1A abolishes the stimulatory effect. Moreover, another p300-dependent promoter, the cAMP-response element, can be superactivated by c-Jun using the same mechanism. These data indicate that c-Jun, when activated or overexpressed, is recruited to the H ferritin promoter by p300, which links NFY, bound to DNA, to the complex. These results add a new level of complexity to transcriptional regulation by c-Jun, which can activate p300-dependent promoters without binding directly to the target DNA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|