Sodium-calcium exchanger is initially expressed in a heart-restricted pattern within the early mouse embryo
| Autor: | Simon J. Conway, Srinagesh V. Koushik, Justin Bundy |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Fetus
Embryology Heartbeat Sodium-calcium exchanger Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation Developmental Heart Single gene Embryo In situ hybridization Biology Molecular biology Sodium-Calcium Exchanger Cell biology Mice Intracellular calcium homeostasis embryonic structures cardiovascular system Animals Developmental Biology |
| Zdroj: | Mechanisms of Development. 88(1):119-122 |
| ISSN: | 0925-4773 |
| DOI: | 10.1016/s0925-4773(99)00177-x |
| Popis: | Although the sodium-calcium exchanger (NCX1) is encoded by a single gene, it is widely expressed in both fetal and adult tissues and functions in many diverse physiological processes to maintain intracellular calcium homeostasis. In order to determine whether NCX1 is also ubiquitously expressed in the early mouse embryo, in situ hybridization and RT-PCR were used to determine the spacio-temporal expression of NCX1. Our results indicate that NCX1 expression is present within the 7.75–8.0 dpc cardiogenic plate before the first heartbeat, and that NCX1 is initially expressed in a heart-restricted pattern within the early mouse embryo. However, in more developed embryos (11.0 dpc and older) NCX1 is expressed in other tissues. |
| Databáze: | OpenAIRE |
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