A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana
| Autor: | Anna-Lena Van de Weyer, Jonathan D. G. Jones, Kamil Witek, Jeffery L. Dangl, Oliver J. Furzer, Detlef Weigel, Volkan Cevik, Freddy Monteiro, Felix Bemm, Marc T. Nishimura |
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| Přispěvatelé: | Gordon and Betty Moore Foundation, Two Blades Foundation, Gatsby Charitable Foundation, Biotechnology and Biological Sciences Research Council (UK), National Science Foundation (US), European Research Council, Max Planck Society |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Arabidopsis
Genomics NLR Proteins SMRT sequencing Plant disease resistance Biology General Biochemistry Genetics and Molecular Biology Article NLR 03 medical and health sciences Plant immunity 0302 clinical medicine Species Specificity genomics Gene family Plant Immunity Allele Gene Pathogen innate immunity Alleles 030304 developmental biology Disease Resistance Plant Diseases Innate immunity Genetics 0303 health sciences Disease resistance genes Innate immune system Arabidopsis Proteins fungi Genetic Variation RenSeq targeted enrichment integrated domains disease resistance genes sequence capture 030217 neurology & neurosurgery Genome Plant Single molecule real time sequencing |
| Zdroj: | Cell Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona Digital.CSIC. Repositorio Institucional del CSIC instname Recercat. Dipósit de la Recerca de Catalunya Recercat: Dipósit de la Recerca de Catalunya Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| ISSN: | 1097-4172 0092-8674 |
| Popis: | Summary Infectious disease is both a major force of selection in nature and a prime cause of yield loss in agriculture. In plants, disease resistance is often conferred by nucleotide-binding leucine-rich repeat (NLR) proteins, intracellular immune receptors that recognize pathogen proteins and their effects on the host. Consistent with extensive balancing and positive selection, NLRs are encoded by one of the most variable gene families in plants, but the true extent of intraspecific NLR diversity has been unclear. Here, we define a nearly complete species-wide pan-NLRome in Arabidopsis thaliana based on sequence enrichment and long-read sequencing. The pan-NLRome largely saturates with approximately 40 well-chosen wild strains, with half of the pan-NLRome being present in most accessions. We chart NLR architectural diversity, identify new architectures, and quantify selective forces that act on specific NLRs and NLR domains. Our study provides a blueprint for defining pan-NLRomes. Graphical Abstract Highlights • Species-wide NLR diversity is high but not unlimited • A large fraction of NLR diversity is recovered with 40–50 accessions • Presence/absence variation in NLRs is widespread, resulting in a mosaic population • A high diversity of NLR-integrated domains favor known virulence targets In plants, NLR proteins are important intracellular receptors with roles in innate immunity and disease resistance. This work provides a panoramic view of this diverse and complicated gene family in the model species A. thaliana and provides a foundation for the identification and functional study of disease-resistance genes in agronomically important species with complex genomes. |
| Databáze: | OpenAIRE |
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