Fermented wheat aleurone enriched with probiotic strains LGG and Bb12 modulates markers of tumor progression in human colon cells
| Autor: | Ursula Obst, Michael Glei, Katrin Stein, Anke Borowicki, Kerstin Scheu, Anke Michelmann, Daniel Scharlau |
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| Rok vydání: | 2010 |
| Předmět: |
Cyclin-Dependent Kinase Inhibitor p21
Dietary Fiber Cancer Research Medicine (miscellaneous) Apoptosis Butyrate Biology chemistry.chemical_compound HT29 Cells Aleurone Biomarkers Tumor Humans RNA Messenger Triticum Cell Proliferation Nutrition and Dietetics Cell growth Probiotics Cell Cycle Fatty Acids Alkaline Phosphatase Molecular biology Receptors TNF-Related Apoptosis-Inducing Ligand Oncology chemistry Biochemistry Cell culture Colonic Neoplasms Fermentation Disease Progression Alkaline phosphatase Growth inhibition |
| Zdroj: | Nutrition and cancer. 63(1) |
| ISSN: | 1532-7914 |
| Popis: | Fermentation of dietary fiber by the microflora enhances the levels of effective metabolites, which are potentially protective against colon cancer. The specific addition of probiotics may enhance the efficiency of fermentation of wheat aleurone, a source of dietary fiber. We investigated the effects of aleurone, fermented with fecal slurries with the addition of the probiotics LGG and Bb12 (aleurone(+)), on cell growth, apoptosis, and differentiation, as well as expression of genes related to growth and apoptosis using two different human colon cell lines (HT29: adenocarcinoma cells; LT97: adenoma cells). The efficiency of fermentation of aleurone was only slightly enhanced by the addition of LGG/Bb12, resulting in an increased concentration of butyrate. In LT97 cells, the growth inhibition of aleurone(+) was stronger than in HT29 cells. In HT29 cells, a cell cycle arrest in G(0)/G(1) and the alkaline phosphatase activity, a marker of differentiation, were enhanced by the fs aleurone(+). Treatment with all fermentation supernatants resulted in a significant increase in apoptosis and an upregulation of genes involved in cell growth and apoptosis (p21 and WNT2B). In conclusion, fs aleurone(+) modulated markers of cancer prevention, namely inhibition of cell growth and promotion of apoptosis as well as differentiation. |
| Databáze: | OpenAIRE |
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