Gene content analysis of sugarcane public ESTs reveals thousands of missing coding-genes and an unexpected pool of grasses conserved ncRNAs

Autor:	M. A. Van Sluys, Fabio Tebaldi Silveira Nogueira, Renato Vicentini, Michel Georges Albert Vincentz, L. E. V. Del Bem
Rok vydání:	2012
Předmět:	Expressed sequence tag business.industry food and beverages Plant Science Computational biology Biology Sorghum biology.organism_classification BIOTECNOLOGIA Biotechnology Transcriptome Sequencing Transcriptome Crop Proteome Genetics Poaceae business Gene
Zdroj:	Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP
Popis:	Sugarcane is the most important crop for sugar industry and raw material for bioethanol. Here we present a quantitative analysis of the gene content from publicly available sugarcane ESTs. The current sugarcane EST collection sampled orthologs for ~58 % of the closely-related sorghum proteome, suggesting that more than 10,000 sugarcane coding-genes remain undiscovered. Moreover the existence of more than 2,000 ncRNAs conserved between sugarcane and sorghum was revealed, among which over 500 are also detected in rice, supporting the existence of hundreds of conserved ncRNAs in grasses. New efforts towards sugarcane transcriptome sequencing were needed to sample the missing coding-genes as well as to expand the catalog of ncRNAs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4331be51bf301864f200dc208cd3de78 Zobrazit plný text záznamu