Hollow-Fiber Enzyme Reactor Operating under Nonisothermal Conditions
| Autor: | Umberto Amato, F.S. Gaeta, Francesca Carfora, Umberto Bencivenga, V. Grano, Maria Lepore, Marianna Portaccio, Damiano Gustavo Mita, Nadia Diano, Sergio Rossi |
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| Přispěvatelé: | Diano, Nadia, Grano, V, Rossi, S, Bencivenga, U, Portaccio, Marianna Bianca Emanuela, Amato, U, Carfora, F, Lepore, Maria, Gaeta, F, Mita, Dg |
| Rok vydání: | 2008 |
| Předmět: |
Materials science
Diamines Catalysis Isothermal process Substrate Specificity chemistry.chemical_compound Bioreactors Bioreactor Coupling (piping) Computer Simulation Fiber Composite material Polypropylene Chromatography Temperature technology industry and agriculture Membranes Artificial Equipment Design Enzymes Immobilized beta-Galactosidase Enzyme Activation Equipment Failure Analysis Temperature gradient Glucose Membrane Models Chemical chemistry Glutaral Hexamethylenediamine Porosity Biotechnology |
| Zdroj: | Biotechnology progress 20 (2004): 457–466. doi:10.1021/bp034197l info:cnr-pdr/source/autori:Diano N.; Grano V.; Rossi S.; Bencivenga U.; Portaccio M.; Amato U.; Carfora F.; Lepore M.; Gaeta F.S.; Mita D.G./titolo:Hollow-fiber enzyme reactor operating under nonisothermal conditions/doi:10.1021%2Fbp034197l/rivista:Biotechnology progress (Print)/anno:2004/pagina_da:457/pagina_a:466/intervallo_pagine:457–466/volume:20 |
| ISSN: | 8756-7938 |
| DOI: | 10.1021/bp034197l |
| Popis: | A hollow-fiber enzyme reactor, operating under isothermal and nonisothermal conditions, was built employing a polypropylene hollow fiber onto which beta-galactosidase was immobilized. Hexamethylenediamine and glutaraldehyde were used as spacer and coupling agent, respectively. Glucose production was studied as a function of temperature, substrate concentration, and size of the transmembrane temperature gradient. The actual average temperature differences across the polypropylene fiber, to which reference was done to evaluate the effect of the nonisothermal conditions, were calculated by means of a mathematical approach, which made it possible to know, using computer simulation, the radial and axial temperature profiles inside the bioreactor and across the membrane. Percent activity increases, proportional to the size of the temperature gradients, were found when the enzyme activities under nonisothermal conditions were compared to those measured under comparable isothermal conditions. Percent reductions of the production times, proportional to the applied temperature gradients, were also calculated. The advantage of employing nonisothermal bioreactors in biotechnological industrial process was discussed. |
| Databáze: | OpenAIRE |
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