Systemic IL-1β production as a consequence of corneal HSV-1 infection-contribution to the development of herpes simplex keratitis
| Autor: | Cillian De Gascun, Jaythoon Hassan, Conor Patrick Malone, Ciaran de Chaumont, Siobhán Smith, Conor Murphy, David Shahnazaryan, Joan Ní Gabhann-Dromgoole, Caroline A. Jefferies |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.diagnostic_test
business.industry Brief Report pathogenesis medicine.medical_treatment peripheral immune response herpes simplex virus type 1 Inflammation medicine.disease Peripheral blood mononuclear cell herpes simplex keratitis Keratitis Pathogenesis Ophthalmology Immune system Cytokine lcsh:Ophthalmology Western blot inflammation lcsh:RE1-994 Immunology medicine medicine.symptom IRF3 business |
| Zdroj: | International Journal of Ophthalmology, Vol 12, Iss 9, Pp 1493-1497 (2019) |
| ISSN: | 2227-4898 2222-3959 |
| DOI: | 10.18240/ijo.2019.09.19 |
| Popis: | This study sought to identify potential therapeutic targets in herpes simplex keratitis (HSK) patients with active and inactive infection by investigating peripheral cytokine production. Peripheral blood mononuclear cells (PBMCs) and serum were prepared from healthy controls and HSK patients during active infection or following treatment (inactive infection). Serum antibody titres were determined by ELISA. Protein expression levels were analysed by Western blot. Cytokine levels were determined by multiplex ELISA. Active corneal herpes simplex virus type 1 (HSV-1) infection resulted in significantly elevated peripheral levels of IL-1β in HSK patients compared to healthy controls, and remained significantly increased following treatment. Elevated production of IL-1β in inactive patients was associated with significantly increased levels of IRF3 and STAT1, key proteins involved in promoting anti-viral immune responses. Our data suggest that inflammation persists beyond the period that it is clinically evident and that enhanced peripheral production of IL-1β may have implications for HSV-1 viral clearance in active and inactive HSK patients. |
| Databáze: | OpenAIRE |
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