Electrochemical Imaging of Cell Activity in Hydrogels Embedded in Grid-shaped Polycaprolactone Scaffolds Using a Large-scale Integration-based Amperometric Device
| Autor: | Noriko Taira, Ryota Kunikata, Yuki Yokokawa, Atsushi Suda, Tomokazu Matsue, Hitoshi Shiku, Yuji Nashimoto, Kosuke Ino |
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| Rok vydání: | 2018 |
| Předmět: |
Cell Survival
Polyesters Cell Culture Techniques Nanotechnology macromolecular substances 02 engineering and technology Electrochemistry 01 natural sciences Analytical Chemistry Mice chemistry.chemical_compound Tissue engineering Animals Cells Cultured Embryonic Stem Cells chemistry.chemical_classification Tissue Engineering Tissue Scaffolds Biomolecule 010401 analytical chemistry technology industry and agriculture Hydrogels Electrochemical Techniques Equipment Design Alkaline Phosphatase 021001 nanoscience & nanotechnology Amperometry Molecular Imaging 0104 chemical sciences Electrochemical imaging Microelectrode chemistry Polycaprolactone Self-healing hydrogels 0210 nano-technology Microelectrodes |
| Zdroj: | Analytical Sciences. 35:39-43 |
| ISSN: | 1348-2246 0910-6340 |
| DOI: | 10.2116/analsci.18sdp01 |
| Popis: | Tissue engineering requires analytical methods to monitor cell activity in hydrogels. Here, we present a method for the electrochemical imaging of cell activity in hydrogels embedded in printed polycaprolactone (PCL) scaffolds. Because a structure made of only hydrogel is fragile, PCL frameworks are used as a support material. A grid-shaped PCL was fabricated using an excluder printer. Photocured hydrogels containing cells were set at each grid hole, and cell activity was monitored using a large-scale integration-based amperometric device. The electrochemical device contains 400 microelectrodes for biomolecule detection, such as dissolved oxygen and enzymatic products. As proof of the concept, alkaline phosphatase and respiration activities of embryonic stem cells in the hydrogels were electrochemically monitored. The results indicate that the electrochemical imaging is useful for evaluating cells in printed scaffolds. |
| Databáze: | OpenAIRE |
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