Combination of polyetherketoneketone scaffold and human mesenchymal stem cells from temporomandibular joint synovial fluid enhances bone regeneration
| Autor: | Monzur Murshed, Zhiguang Zhang, Mayumi Umebayashi, Simon D. Tran, Guoying Dong, Yaoyao Fiona Zhao, Michael Roskies, Mohamed-Nur Abdallah, Yi Lin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
endocrine system Scaffold Bone Regeneration Polymers medicine.medical_treatment lcsh:Medicine Bone grafting Article Immunophenotyping Benzophenones 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tissue engineering Osteogenesis In vivo Synovial Fluid medicine Animals Humans Synovial fluid lcsh:Science Bone regeneration Cells Cultured Multidisciplinary Temporomandibular Joint Tissue Engineering Tissue Scaffolds lcsh:R Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Polyetherketoneketone Disease Models Animal 030104 developmental biology chemistry lcsh:Q Rabbits Biomarkers 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-018-36778-2 |
| Popis: | Therapies using human mesenchymal stem cells (MSCs) combined with three-dimensional (3D) printed scaffolds are a promising strategy for bone grafting. But the harvest of MSCs still remains invasive for patients. Human synovial fluid MSCs (hSF-MSCs), which can be obtained by a minimally invasive needle-aspiration procedure, have been used for cartilage repair. However, little is known of hSF-MSCs in bone regeneration. Polyetherketoneketone (PEKK) is an attractive bone scaffold due to its mechanical properties comparable to bone. In this study, 3D-printed PEKK scaffolds were fabricated using laser sintering technique. hSF-MSCs were characterized and cultured on PEKK to evaluate their cell attachment, proliferation, and osteogenic potential. Rabbit calvarial critical-sized bone defects were created to test the bone regenerative effect of PEKK with hSF-MSCs. In vitro results showed that hSF-MSCs attached, proliferated, and were osteogenic on PEKK. In vivo results indicated that PEKK seeded with hSF-MSCs regenerated twice the amount of newly formed bone when compared to PEKK seeded with osteogenically-induced hSF-MSCs or PEKK scaffolds alone. These results suggested that there was no need to induce hSF-MSCs into osteoblasts prior to their transplantations in vivo. In conclusion, the combined use of PEKK and hSF-MSCs was effective in regenerating critical-sized bone defects. |
| Databáze: | OpenAIRE |
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