Activated T-cells and pro-inflammatory cytokines differentially regulate prostaglandin E2 secretion by mesenchymal stem cells
| Autor: | Éva Monostori, Ferenc Uher, Gyöngyi Kudlik, Beáta Hegyi |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Stromal cell T-Lymphocytes Biophysics Bone Marrow Cells Cell Communication Biology Lymphocyte Activation Nitric Oxide Biochemistry Dinoprostone Proinflammatory cytokine Interferon-gamma Mice Immune system Internal medicine medicine Animals Secretion Prostaglandin E2 Molecular Biology Cells Cultured Tumor Necrosis Factor-alpha Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology Coculture Techniques Recombinant Proteins Cell biology Mice Inbred C57BL Endocrinology medicine.anatomical_structure Adipose Tissue Cyclooxygenase 2 Cytokines Tumor necrosis factor alpha Bone marrow medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 419:215-220 |
| ISSN: | 0006-291X |
| Popis: | In recent years it has become clear that mesenchymal stem or stromal cells (MSCs) are capable of modulating inflammatory and immune responses through interaction with a wide variety of cells. Whereas several studies indicated that PGE2 is one of the chief soluble mediators involved in these processes, here we investigated prostaglandin E2 (PGE2) production of murine bone marrow- (BM-) and adipose tissue- (Ad-) derived MSCs stimulated with pro-inflammatory cytokines TNF-α and IFN-γ, or co-cultured with ConA-induced T-cell blasts. We found that both MSC populations are able to produce high amounts of PGE2 in MSC/activated T-cell co-cultures. This effect was markedly attenuated when direct cell-cell contact was prevented in transwell system, indicating that the elicitation of the PGE2 secretion of MSCs is contact-dependent in this experimental setting. In contrast, when soluble recombinant pro-inflammatory cytokines were added to the MSC cultures, TNF-α and IFN-γ act synergistically to induce PGE2 production, whereas only high amount of TNF-α but not IFN-γ was able to do so alone. Although the PGE2 secretion by MSCs was completely abrogated by addition of indomethacin under all culture conditions tested, L-NMA, a NOS inhibitor could only partially inhibit it when the cells were elicited in the concomitant presence of TNF-α and IFN-γ. These results, combined with others, suggest that NO acts downstream of IFN-γ but upstream of COX2. Taken together, our findings demonstrate that the induction of PGE2 secretion by BM- and Ad-MSCs is not mediated by a single or unique, nonredundant molecular mechanism under different experimental conditions. |
| Databáze: | OpenAIRE |
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