Immunohistochemical Staging of Neurofibrillary Degeneration in Alzheimerʼs Disease
| Autor: | Douglas L. Hauser, Claude M. Wischik, William Bondareff, Martin Roth, Charles R. Harrington |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pathology
medicine.medical_specialty tau Proteins Biology Hippocampus Antibodies Epitope Pathology and Forensic Medicine Epitopes Cellular and Molecular Neuroscience Alzheimer Disease Microtubule Extracellular medicine Humans Microscopy Neurofibrillary Tangles Neurofibrillary tangle General Medicine medicine.disease Immunohistochemistry Xanthenes Neurology Pronase Nerve Degeneration biology.protein Neurology (clinical) Alzheimer's disease Antibody Fluorescein-5-isothiocyanate Intracellular |
| Zdroj: | Journal of Neuropathology and Experimental Neurology. 53:158-164 |
| ISSN: | 0022-3069 |
| DOI: | 10.1097/00005072-199403000-00007 |
| Popis: | Antibodies to different phosphorylated and non-phosphorylated tau epitopes have been used to identify three histologically distinct types of neurofibrillary tangles in Alzheimer's disease. Intracellular tangles (Type 1) were identified by antibodies recognizing epitopes throughout the tau molecule, including the NH2-terminus. Compact extracellular tangles (Type 2) were characterized by the loss of NH2-terminal immunoreactivity and retention of other tau epitopes. Dispersed extracellular tangles (Type 3) were characterized by the presence of epitopes associated with the microtubule binding region and the COOH-terminus. These three types of tangles, found in situ in hippocampus, could be created experimentally by proteolytic treatment of brain sections. These findings suggest that three stages of neurofibrillary degeneration can be understood as a sequential stripping of paired helical filaments in which the loss of amino-terminus epitopes, followed by loss of phosphorylated epitopes, results in the appearance of dispersed extracellular tangles containing PHF-core epitopes. |
| Databáze: | OpenAIRE |
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