Distinguishing angioimmunoblastic T-cell lymphoma from peripheral T-cell lymphoma, unspecified, using morphology, immunophenotype and molecular genetics
Autor: | PG Isaacson, Ahmet Dogan, Ming-Qing Du, S. S Chuang, TC Diss, Ayoma D. Attygalle |
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Rok vydání: | 2007 |
Předmět: |
Herpesvirus 4
Human Angioimmunoblastic T-cell lymphoma Pathology medicine.medical_specialty Histology CD3 Complex T-Lymphocytes Biology Polymerase Chain Reaction Sensitivity and Specificity Immunophenotyping Pathology and Forensic Medicine Diagnosis Differential immune system diseases hemic and lymphatic diseases medicine Humans Anaplastic large-cell lymphoma Gene Rearrangement CD20 B-Lymphocytes Follicular dendritic cells Lymphoma T-Cell Peripheral Receptors Antigen T-Cell gamma-delta General Medicine Gene rearrangement Antigens CD20 medicine.disease Immunohistochemistry Peripheral T-cell lymphoma Clone Cells Lymphoma Immunoblastic Lymphadenopathy embryonic structures biology.protein RNA Viral Neprilysin Receptors Complement 3d Immunoglobulin Heavy Chains |
Zdroj: | Histopathology. 50:498-508 |
ISSN: | 1365-2559 0309-0167 |
Popis: | Distinguishing angioimmunoblastic T-cell lymphoma from peripheral T-cell lymphoma, unspecified, using morphology, immunophenotype and molecular genetics Aims: To identify distinguishing histological, immunophenotypic and molecular genetic features between angioimmunoblastic T-cell lymphoma (AITL) and peripheral T-cell lymphoma (PTL). Methods: Nodal T-cell lymphomas examined (n =137), included AITL (n = 89), PTL (n = 22), anaplastic large cell lymphoma (n = 16) and 'AITL/PTL indeterminate' (n = 10) with overlapping features between AITL and PTL, showing morphology typical of AITL but lacking follicular dendritic cell expansion. Immunohistochemistry for CD3, CD20, CD21 and CD10, in situ hybridization for Epstein-Barr virus encoded RNA (EBER) and polymerase chain reaction for T-cell and B-cell clonality analysis were performed. Results: Of the AITLs, 74/89 showed typical morphology, whereas 15/89 showed hyperplastic follicles. AITL and 'AITL/PTL indeterminate' showed a polymorphous infiltrate and prominent vascularity in all cases. In both groups, CD10 was present in the majority and clear cells and EBER positivity were specific (but not universal) features lacking in PTL. Detection of T-cell clonality was significantly higher in AITL (90%) compared with PTLu (59%). Conclusions: Clear cells and EBV infection (when present) are useful distinguishing features and CD10 a sensitive and specific marker of AITL. Hyperplastic follicles are present in a significant minority of AITL. AITL/PTL indeterminate probably falls within the spectrum of AITL rather than PTL. |
Databáze: | OpenAIRE |
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