Use of semiconductor-based oligonucleotide microarrays for influenza a virus subtype identification and sequencing
| Autor: | Michael J. Lodes, Kim Dix, Richard J. Webby, Wanda J. Lyon, Dominic Suciu, Axel G. Stöver, Mark Elliott, David Danley, Marcelo Caraballo, James Crye, Marty Ross, Andrew McShea |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Gene Expression Regulation Viral biology Gene Expression Profiling Orthomyxoviridae Virulence Hemagglutinin (influenza) Neuraminidase Hemagglutinin Glycoproteins Influenza Virus Sequence Analysis DNA biology.organism_classification medicine.disease_cause Virology Genome Virus Gene expression profiling Semiconductors Influenza A virus biology.protein medicine Oligonucleotide Array Sequence Analysis |
| Zdroj: | Journal of clinical microbiology. 44(4) |
| ISSN: | 0095-1137 |
| Popis: | In the face of concerns over an influenza pandemic, identification of virulent influenza A virus isolates must be obtained quickly for effective responses. Rapid subtype identification, however, is difficult even in well-equipped virology laboratories or is unobtainable in the field under more austere conditions. Here we describe a genome assay and microarray design that can be used to rapidly identify influenza A virus hemagglutinin subtypes 1 through 15 and neuraminidase subtypes 1 through 9. Also described is an array-based enzymatic assay that can be used to sequence portions of both genes or any other sequence of interest. |
| Databáze: | OpenAIRE |
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