Amyloid precursor protein traffics from the Golgi directly to early endosomes in an Arl5b- and AP4-dependent pathway
| Autor: | Khalisah L Zulkefli, Fiona J. Houghton, Jing Zhi A. Tan, Wei Hong Toh, Paul A. Gleeson |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Endosome Golgi Apparatus Endosomes Biology Biochemistry Cell membrane Amyloid beta-Protein Precursor 03 medical and health sciences symbols.namesake 0302 clinical medicine Structural Biology Cell Line Tumor mental disorders Genetics medicine Amyloid precursor protein Humans Secretion Transport Vesicles Molecular Biology Amyloid beta-Peptides ADP-Ribosylation Factors Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Cell Membrane RNA-Binding Proteins Cell Biology Membrane transport Golgi apparatus Transport protein Cell biology DNA-Binding Proteins Protein Transport 030104 developmental biology medicine.anatomical_structure symbols biology.protein Amyloid Precursor Protein Secretases Lysosomes Protein Processing Post-Translational Amyloid precursor protein secretase 030217 neurology & neurosurgery HeLa Cells trans-Golgi Network |
| Zdroj: | Traffic. 18:159-175 |
| ISSN: | 1398-9219 |
| Popis: | The intracellular trafficking and proteolytic processing of the membrane-bound amyloid precursor protein (APP) are coordinated events leading to the generation of pathogenic amyloid-beta (Aβ) peptides. The membrane transport of newly synthesized APP from the Golgi to the endolysosomal system is not well defined, yet it is likely to be critical for regulating its processing by β-secretase (BACE1) and γ-secretase. Here, we show that the majority of newly synthesized APP is transported from the trans-Golgi network (TGN) directly to early endosomes and then subsequently to the late endosomes/lysosomes with very little transported to the cell surface. We show that Arl5b, a small G protein localized to the TGN, and AP4 are essential for the post-Golgi transport of APP to early endosomes. Arl5b is physically associated with AP4 and is required for the recruitment of AP4, but not AP1, to the TGN. Depletion of either Arl5b or AP4 results in the accumulation of APP, but not BACE1, in the Golgi, and an increase in APP processing and Aβ secretion. These findings demonstrate that APP is diverted from BACE1 at the TGN for direct transport to early endosomes and that the TGN represents a site for APP processing with the subsequent secretion of Aβ. |
| Databáze: | OpenAIRE |
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