Focused ultrasound and microbubbles for enhanced extravasation
| Autor: | Ceciel Chlon, Alexander L. Klibanov, Marcel Rene Bohmer, Talent I. Shevchenko, Chien Ting Chin, Balasundara Raju |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Muscle tissue
medicine.medical_specialty Pharmaceutical Science Vascular permeability 02 engineering and technology Article Capillary Permeability Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo medicine Animals Ultrasonics Particle Size Evans Blue Microbubbles business.industry Muscles Ultrasound Albumin 021001 nanoscience & nanotechnology Extravasation Surgery Mice Inbred C57BL medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Female 0210 nano-technology business Gels Biomedical engineering |
| Zdroj: | Journal of Controlled Release |
| ISSN: | 0168-3659 |
| DOI: | 10.1016/j.jconrel.2010.06.012 |
| Popis: | The permeability of blood vessels for albumin can be altered by using ultrasound and polymer or lipid-shelled microbubbles. The region in which the microbubbles were destroyed with focused ultrasound was quantified in gel phantoms as a function of pressure, number of cycles and type of microbubble. At 2 MPa the destruction took place in a fairly wide area for a lipid-shelled agent, while for polymer-shelled agents at this setting, distinct destruction spots with a radius of only 1 mm were obtained. When microbubbles with a thicker shell were used, the pressure above which the bubbles were destroyed shifts to higher values. In vivo both lipid and polymer microbubbles increased the extravasation of the albumin binding dye Evans Blue, especially in muscle leading to about 6–8% of the injected dose to extravasate per gram muscle tissue 30 minutes after start of the treatment, while no Evans Blue could be detected in muscle in the absence of microbubbles. Variation in the time between ultrasound treatment and Evans Blue injection, demonstrated that the time window for promoting extravasation is at least an hour at the settings used. In MC38 tumors, extravasation already occurred without ultrasound and only a trend towards enhancement with about a factor 2 could be established with a maximum percentage injected dose per gram of 3%. Ultrasound mediated microbubble destruction especially enhances the extravasation in the highly vascularized outer part of the MC38 tumor and adjacent muscle and would, therefore, be most useful for release of, for instance, anti-angiogenic drugs. |
| Databáze: | OpenAIRE |
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