Differential Macrophage Polarization Promotes Tissue Remodeling and Repair in a Model of Ischemic Retinopathy
| Autor: | Samia N. Naccache, Valentina Marchetti, Kathleen Storm, Edith Aguilar, Gary Siuzdak, Glen R. Nemerow, Stacey K. Moreno, Martin Friedlander, Oscar Yanes, M. Wang, David F. Friedlander, Min Zhan |
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| Rok vydání: | 2011 |
| Předmět: |
Pathology
medicine.medical_specialty Angiogenesis Lipopolysaccharide Receptors Macrophage polarization Biology Polymerase Chain Reaction Article Mice 03 medical and health sciences 0302 clinical medicine Retinal Diseases Ischemia medicine Animals Humans Cells Cultured 030304 developmental biology 0303 health sciences Microscopy Confocal Multidisciplinary Extramural Macrophages Retinal Vessels 3. Good health Disease Models Animal Tissue remodeling Cancer research Ischemic retinopathy Stem cell 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Diabetic retinopathy is the leading cause of visual loss in individuals under the age of 55. Umbilical cord blood (UCB)-derived myeloid progenitor cells have been shown to decrease neuronal damage associated with ischemia in the central nervous system. In this study we show that UCB-derived CD14(+) progenitor cells provide rescue effects in a mouse model of ischemic retinopathy by promoting physiological angiogenesis and reducing associated inflammation. We use confocal microscopy to trace the fate of injected human UCB-derived CD14(+) cells and PCR with species-specific probes to investigate their gene expression profile before and after injection. Metabolomic analysis measures changes induced by CD14(+) cells. Our results demonstrate that human cells differentiate in vivo into M2 macrophages and induce the polarization of resident M2 macrophages. This leads to stabilization of the ischemia-injured retinal vasculature by modulating the inflammatory response, reducing oxidative stress and apoptosis and promoting tissue repair. |
| Databáze: | OpenAIRE |
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