Comparative Analysis of Membrane Vesicles from Three Piscirickettsia salmonis Isolates Reveals Differences in Vesicle Characteristics

Autor: Hanne C. Winther-Larsen, Petter Langlete, Julia Isabel Tandberg, Deepa Varkey, Norbert Roos, Leidy Lagos, Ian T. Paulsen, Eva Berger, Anne-Lise Rishovd
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Proteomics
Cell Membranes
lcsh:Medicine
Pathology and Laboratory Medicine
Biochemistry
Mass Spectrometry
Piscirickettsia
Piscirickettsia salmonis
Medicine and Health Sciences
Chile
lcsh:Science
Immune Response
Zebrafish
Multidisciplinary
biology
Norway
Vesicle
Fishes
Animal Models
Bacterial Pathogens
Osteichthyes
Medical Microbiology
Proteome
Vertebrates
Cellular Structures and Organelles
Pathogens
Salmonidae
Research Article
Canada
Gram-negative bacteria
General Science & Technology
Virulence Factors
Immunology
Virulence
Research and Analysis Methods
Microbiology
03 medical and health sciences
Model Organisms
Bacterial Proteins
DNA-binding proteins
Animals
Vesicles
Microbial Pathogens
lcsh:R
Cytoplasmic Vesicles
Biofilm
Organisms
Biology and Life Sciences
Membrane Proteins
Proteins
Cell Biology
biology.organism_classification
Outer Membrane Proteins
Chaperone Proteins
030104 developmental biology
Piscirickettsiaceae Infections
lcsh:Q
Bacteria
Zdroj: PLoS ONE, Vol 11, Iss 10, p e0165099 (2016)
PLoS ONE
ISSN: 1932-6203
Popis: Membrane vesicles (MVs) are spherical particles naturally released from the membrane of Gram-negative bacteria. Bacterial MV production is associated with a range of phenotypes including biofilm formation, horizontal gene transfer, toxin delivery, modulation of host immune responses and virulence. This study reports comparative profiling of MVs from bacterial strains isolated from three widely disperse geographical areas. Mass spectrometry identified 119, 159 and 142 proteins in MVs from three different strains of Piscirickettsia salmonis isolated from salmonids in Chile (LF-89), Norway (NVI 5692) and Canada (NVI 5892), respectively. MV comparison revealed several strain-specific differences related to higher virulence capability for LF-89 MVs, both in vivo and in vitro, and stronger similarities between the NVI 5692 and NVI 5892 MV proteome. The MVs were similar in size and appearance as analyzed by electron microscopy and dynamic light scattering. The MVs from all three strains were internalized by both commercial and primary immune cell cultures, which suggest a potential role of the MVs in the bacterium’s utilization of leukocytes. When MVs were injected into an adult zebrafish infection model, an upregulation of several pro-inflammatory genes were observed in spleen and kidney, indicating a modulating effect on the immune system. The present study is the first comparative analysis of P. salmonis derived MVs, highlighting strain-specific vesicle characteristics. The results further illustrate that the MV proteome from one bacterial strain is not representative of all bacterial strains within one species.
Databáze: OpenAIRE
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