Mutagenicity and genotoxicity of PM2.5 issued from an urbano-industrialized area of Dunkerque (France)

Autor: J. Le Goff, Guillaume Garçon, François Sichel, P. Shirali, Véronique André, S. Billet, Ivannah Pottier, Didier Pottier
Přispěvatelé: Groupe Régional d'Etudes sur le CANcer (GRECAN), IFR146-Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale (ULCO), Université de Caen Normandie (UNICAEN), Centre Régional de Lutte contre le Cancer François Baclesse [Caen] (UNICANCER/CRLC), UNICANCER-Tumorothèque de Caen Basse-Normandie (TCBN)-Normandie Université (NU)
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Salmonella typhimurium
Time Factors
Primary culture
MESH: Macrophages
Alveolar
[SDV]Life Sciences [q-bio]
MESH: Mutagenicity Tests
010501 environmental sciences
Toxicology
medicine.disease_cause
01 natural sciences
MESH: Carcinogens
Ames test
DNA Adducts
Cells
Cultured
0303 health sciences
Chemistry
Particulates
3. Good health
MESH: Particulate Matter
8-Hydroxy-2'-Deoxyguanosine
Environmental chemistry
France
MESH: DNA Adducts
MESH: Cells
Cultured
Chronic exposure
Carcinogenicity Tests
MESH: Deoxyguanosine
Cell Line
03 medical and health sciences
Species Specificity
MESH: Mutagens
Macrophages
Alveolar
medicine
Humans
Industry
Aerodynamic diameter
Carcinogenicity Test
MESH: Particle Size
Particle Size
030304 developmental biology
0105 earth and related environmental sciences
A549 cell
MESH: Humans
Mutagenicity Tests
Osmolar Concentration
Urbanization
Deoxyguanosine
MESH: Salmonella typhimurium
MESH: Carcinogenicity Tests
Carcinogens
Particulate Matter
MESH: Industry
Genotoxicity
Mutagens
Zdroj: Journal of Applied Toxicology
Journal of Applied Toxicology, Wiley, 2011, 31 (2), pp.131-138. ⟨10.1002/jat.1572⟩
ISSN: 0260-437X
1099-1263
DOI: 10.1002/jat.1572⟩
Popis: International audience; Epidemiological studies have demonstrated the link between chronic exposure to particulate matter (PM), especially particles with an aerodynamic diameter lesser than 2.5 µm (PM(2.5) ), and lung cancer. Mechanistic investigations focus on the contribution of the various genotoxicants adsorbed onto the particles, and more particularly on polycyclic aromatic hydrocarbons or nitroaromatics. Most of the previous studies dealing with genotoxic and/or mutagenic measurements were performed on organic extracts obtained from PM(2.5) collected in polluted areas. In contrast, we have evaluated genotoxic and mutagenic properties of urbano-industrial PM(2.5) (PM) collected in Dunkerque (France). Thermally desorbed PM(2.5) (dPM) was also comparatively studied. Suspensions of PM and dPM (5-50 µg per plate) were tested in Salmonella tester strains TA98, TA102 and YG1041 ± S9mix. Significant mutagenicity was observed for PM in YG1041 ± S9 mix. In strain TA102 - S9mix, a slight, but not significant dose-response increase was observed, for both PM and dPM. Genotoxic properties of PM and dPM were evaluated by the measurement of (1) 8-OHdG in A549 cells and (2) bulky DNA adducts on A549 cells and on human alveolar macrophages (AMs) in primary culture. A dose-dependant formation of 8-OHdG adducts was observed on A549 cells for PM and dPM, probably mainly attributed to the core of the particles. Bulky DNA adducts were observed only in AMs after exposure to PM and dPM. In conclusion, using relevant exposure models, suspension of PM(2.5) induces a combination of DNA-interaction mechanisms, which could contribute to the induction of lung cancer in exposed populations.
Databáze: OpenAIRE
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