Small Regulatory RNAs of Rickettsia conorii
| Autor: | Kamil Khanipov, Yuriy Fofanov, Sanjeev K. Sahni, Hema P. Narra, Abha Sahni, Mark Rojas, Casey L. C. Schroeder |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Ixodidae Sequence analysis Gene Expression Disease Vectors Biology Boutonneuse Fever Article Bacterial genetics 03 medical and health sciences Consensus Sequence Gene expression Consensus sequence Animals Humans Gene Cells Cultured Genetics Regulation of gene expression Multidisciplinary Base Sequence Sequence Analysis RNA Endothelial Cells RNA Gene Expression Regulation Bacterial biology.organism_classification 3. Good health RNA Bacterial Rickettsia conorii 030104 developmental biology Riboswitch RNA Small Untranslated |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep36728 |
| Popis: | Small regulatory RNAs comprise critically important modulators of gene expression in bacteria, yet very little is known about their prevalence and functions in Rickettsia species. R. conorii, the causative agent of Mediterranean spotted fever, is a tick-borne pathogen that primarily infects microvascular endothelium in humans. We have determined the transcriptional landscape of R. conorii during infection of Human Microvascular Endothelial Cells (HMECs) by strand-specific RNA sequencing to identify 4 riboswitches, 13 trans-acting (intergenic), and 22 cis-acting (antisense) small RNAs (termed ‘Rc_sR’s). Independent expression of four novel trans-acting sRNAs (Rc_sR31, Rc_sR33, Rc_sR35, and Rc_sR42) and known bacterial sRNAs (6S, RNaseP_bact_a, ffs, and α-tmRNA) was next confirmed by Northern hybridization. Comparative analysis during infection of HMECs vis-à-vis tick AAE2 cells revealed significantly higher expression of Rc_sR35 and Rc_sR42 in HMECs, whereas Rc_sR31 and Rc_sR33 were expressed at similar levels in both cell types. We further predicted a total of 502 genes involved in all important biological processes as potential targets of Rc_sRs and validated the interaction of Rc_sR42 with cydA (cytochrome d ubiquinol oxidase subunit I). Our findings constitute the first evidence of the existence of post-transcriptional riboregulatory mechanisms in R. conorii and interactions between a novel Rc_sR and its target mRNA. |
| Databáze: | OpenAIRE |
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