Microsatellite instability (MSI) increases with age in normal somatic cells
| Autor: | Louis S. Ramagli, Jingping Xu, Mary Coolbaugh-Murphy, Michael J. Siciliano, Barry W. Brown |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Aging Somatic cell DNA Mutational Analysis Quantitative Trait Loci Mutant Biology Polymerase Chain Reaction law.invention law medicine Humans Polymerase chain reaction Aged DNA Sequence Unstable nutritional and metabolic diseases Microsatellite instability Middle Aged medicine.disease Phenotype Molecular biology digestive system diseases Ageing Mutation Microsatellite Female DNA mismatch repair Microsatellite Repeats Developmental Biology |
| Zdroj: | Mechanisms of Ageing and Development. 126:1051-1059 |
| ISSN: | 0047-6374 |
| DOI: | 10.1016/j.mad.2005.06.005 |
| Popis: | Small pool PCR (SP-PCR) is a sensitive method for the detection and quantification of microsatellite instability (MSI) in somatic cells. Here we propose that mutant microsatellite fragments accumulate with age in normal somatic cells and that this increase in MSI can be quantified by SP-PCR. MSI at 6 microsatellite loci was determined by SP-PCR in PBL DNA from 17 "normal" blood bank donors. These individuals varied in age from 20 to 67 y/o. MSI phenotypes were plotted against age in a regression analyses. A positive slope indicated a correlation between age and MSI phenotype (p=0.0006). The mean weighted average mutant frequencies across all loci for all individuals in the age groups (0.009 for 20-30 y/o; 0.019 for 35-50 y/o; 0.034 for 60-70 y/o) were also significantly different from each other (p0.01). A baseline for increases of MSI with age in human somatic cells was therefore begun and the effectiveness of SP-PCR to evaluate low, but significant, levels of MSI, established. |
| Databáze: | OpenAIRE |
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