Proper Sterol Distribution Is Required for Candida albicans Hyphal Formation and Virulence
| Autor: | Melissa Donigan, Hsing-Yin Liu, Adam Bata, Josie E. Parker, Joseph T. Nickels, Steven L. Kelly, Caroline Giordano, Paula McCourt, Christina Gallo-Ebert |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Hypha 030106 microbiology Mutant Virulence Investigations QH426-470 Biology biology.organism_classification Phenotype Homology (biology) Corpus albicans Sterol Microbiology virulence 03 medical and health sciences sterol 030104 developmental biology lipid Genetics hyphae Candida albicans Molecular Biology Genetics (clinical) Candida |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 6, Iss 11, Pp 3455-3465 (2016) G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.116.033969 |
| Popis: | Candida albicans is an opportunistic fungus responsible for the majority of systemic fungal infections. Multiple factors contribute to C. albicans pathogenicity. C. albicans strains lacking CaArv1 are avirulent. Arv1 has a conserved Arv1 homology domain (AHD) that has a zinc-binding domain containing two cysteine clusters. Here, we explored the role of the CaAHD and zinc-binding motif in CaArv1-dependent virulence. Overall, we found that the CaAHD was necessary but not sufficient for cells to be virulent, whereas the zinc-binding domain was essential, as Caarv1/Caarv1 cells expressing the full-length zinc-binding domain mutants, Caarv1C3S and Caarv1C28S, were avirulent. Phenotypically, we found a direct correlation between the avirulence of Caarv1/Caarv1, Caarrv1AHD, Caarv1C3S, and Caarv1C28S cells and defects in bud site selection, septa formation and localization, and hyphal formation and elongation. Importantly, all avirulent mutant strains lacked the ability to maintain proper sterol distribution. Overall, our results have established the importance of the AHD and zinc-binding domain in fungal invasion, and have correlated an avirulent phenotype with the inability to maintain proper sterol distribution. |
| Databáze: | OpenAIRE |
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