Differential gene expression profiling of one- and two-dimensional apogamous gametophytes of the fern Dryopteris affinis ssp. affinis
| Autor: | Stefan Wyder, H. Fernández, Ueli Grossniklaus, Valeria Gagliardini, Alejandro Rivera, Ana Elisa Valdés, María Jesús Cañal |
|---|---|
| Přispěvatelé: | University of Zurich, Fernández, Helena |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Dryopteris Physiology Meristem growth Asexual reproduction Plant Science 580 Plants (Botany) Biology Genes Plant 01 natural sciences UFSP13-7 Evolution in Action: From Genomes to Ecosystems 03 medical and health sciences 10126 Department of Plant and Microbial Biology 1311 Genetics Gene Expression Regulation Plant Apomixis 1110 Plant Science Genetics 10211 Zurich-Basel Plant Science Center Gametophyte Gene Expression Profiling Dryopteris affinis 1314 Physiology biology.organism_classification Sexual reproduction 030104 developmental biology Fern Germ Cells Plant Prothallium 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry. 148:302-311 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2020.01.021 |
| Popis: | Apomixis was originally defined as the replacement of sexual reproduction by an asexual process that does not involve fertilization but, in angiosperms, it is often used in the more restricted sense of asexual reproduction through seeds. In ferns, apomixis combines the production of unreduced spores (diplospory) and the formation of sporophytes from somatic cells of the prothallium (apogamy). The genes that control the onset of apogamy in ferns are largely unknown. In this study, we describe the gametophyte transcriptome of the apogamous fern Dryopteris affinis ssp. affinis using an RNA-Seq approach to compare the gene expression profiles of one- and two-dimensional gametophytes, the latter containing apogamic centers. After collapsing highly similar de novo transcripts, we obtained 166,191 unigenes, of which 30% could be annotated using public databases. Multiple quality metrics indicate a good quality of the de novo transcriptome with a low level of fragmentation. Our data show a total of 10,679 genes (6% of all genes) to be differentially expressed between gametophytes of filamentous (one-dimensional) and prothallial (two-dimensional) architecture. 6,110 genes were up-regulated in two-dimensional relative to one-dimensional gametophytes, some of which are implicated in the regulation of meristem growth, auxin signaling, reproduction, and sucrose metabolism. 4,570 genes were down-regulated in two-dimensional versus one-dimensional gametophytes, which are enriched in stimulus and defense genes, as well as genes involved in epigenetic gene regulation and ubiquitin degradation. Our results provide insights into free-living gametophyte development, focusing on the filamentous-to-prothallus growth transition, and provide a useful resource for further investigations of asexual reproduction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect