CampylobacterExcreted into the Environment by Animal Sources: Prevalence, Concentration Shed, and Host Association
| Autor: | Anne Patricia Thomson, Marion MacRae, Norval J. C. Strachan, John F. Dallas, Ovidiu Rotariu, Malcolm J. B. Leitch, Kenny W Reay, Martin C. J. Maiden, Ken J. Forbes, Iain D Ogden, Samuel K. Sheppard |
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| Rok vydání: | 2009 |
| Předmět: |
Disease reservoir
Colony Count Microbial Animals Wild Campylobacter coli medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Campylobacter jejuni Article Birds Foodborne Diseases Feces Species Specificity medicine Animals Phylogeny Disease Reservoirs Bacterial Shedding Models Genetic biology Host (biology) Animal Sources Campylobacter biology.organism_classification Bacterial Typing Techniques Cross-Sectional Studies Haplotypes Scotland Animals Domestic Host-Pathogen Interactions Animal Science and Zoology Host specificity Food Science |
| Popis: | An intensive study of 443 isolates of Campylobacter jejuni and Campylobacter coli from 2031 fecal samples excreted by animal sources including cattle, sheep, and pigs, a range of wild and domesticated avian species and pets is described. The prevalence found in the majority of animal sources ranged from 22% to 28% with poultry being highest at 41% and cats and dogs lowest (5%). The average count excreted for each animal source was found not to be significantly different ranging from approximately 10(2) to 10(5) cfu/g. Multilocus sequence typing (MLST) identified phylogenies that exhibited host specificity. A number of clonal complexes (CCs) and sequence types (STs) were characteristic of particular hosts (e.g., CC-179, ST-637, and ST-1341 found only in pigeons and gulls). Analysis of genetic distance demonstrated numerous significant differences in the distribution of MLST types (CC, ST, and allele) between animal sources. Host association was quantified using structure that correctly assigned the nine animal sources with accuracies of 28%, 24%, and 55% at the CC, ST, and allele levels, respectively. This is substantially higher than would be expected by random allocation (11%) but farmyard poultry had the lowest assignment accuracy (13%, 13%, and 21%) suggesting that isolates were shared with a wide range of other animals. This study demonstrates the link between MLST type and host and provides data that can be used in risk assessment and food attribution models. Further, it demonstrates the applicability of MLST to characterize Campylobacter strains from a broad range of environmental sources. |
| Databáze: | OpenAIRE |
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