Molecular alterations of newly formed mandibular bone caused by zoledronate
| Autor: | Eiki Yamachika, Yasuhiro Kobayashi, Makoto Nakanishi, Masakazu Matsubara, Tadashi Ninomiya, Kazuki Nakatsuji, Seiji Iida, Norifumi Moritani, Tatsuo Fujii, Y. Yoshioka |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Ovariectomy 030209 endocrinology & metabolism Mandible Matrix (biology) Spectrum Analysis Raman Zoledronic Acid 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine Bone Density Internal medicine Bone quality medicine Animals Femur Rats Wistar Bone Density Conservation Agents business.industry Structural integrity X-Ray Microtomography medicine.disease Rats Raman microspectroscopy 030104 developmental biology Endocrinology Otorhinolaryngology Ovariectomized rat Female Surgery Oral Surgery Osteonecrosis of the jaw business Biomarkers Mandibular ramus |
| Zdroj: | International Journal of Oral and Maxillofacial Surgery. 47:1206-1213 |
| ISSN: | 0901-5027 |
| Popis: | Bone quality is defined by structural and material characteristics. Most studies on the mandible have focused on the analysis of structural characteristics, with insufficient investigation of material characteristics. This study tested whether zoledronate affects the material characteristics of newly formed mandibular bone. Thirty-six female Wistar rats were assigned to three groups: sham-ovariectomized rats (SHAM, n=12), ovariectomized rats (OVX, n=12), and ovariectomized rats treated with zoledronate (ZOL, n=12). The left side of the mandibular ramus of all rats was drilled bicortically. Twenty-eight days after surgery, all surviving rats were euthanized and all mandibles were removed. Raman microspectroscopy was performed, and five spectra per specimen of newly formed mandibular bone were analysed. Compared with OVX rats, the mineral/matrix ratio in ZOL rats was significantly increased (5.43±1.88 vs. 7.86±2.05), while crystallinity (0.055±0.002 vs. 0.050±0.002), relative proteoglycan content (0.43±0.10 vs. 0.31±0.05), and collagen structural integrity (1.16±0.21 vs. 0.72±0.06) were significantly decreased. These changes in material characteristics may explain why rats that received zoledronate exhibited peculiar biological phenomena such as bisphosphonate-related osteonecrosis of the jaw. |
| Databáze: | OpenAIRE |
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