Identification of Antigenic Epitopes of 1D Antigen Recognized by Antibodies in the Serum of Patients with Thyroid-Associated Ophthalmopathy
| Autor: | Z.-G. Zhang, N. F. Bernard, J. R. Wall |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Antigenicity DNA Complementary Eye Diseases Molecular Sequence Data Immunology Autoimmunity Biology medicine.disease_cause Thyroiditis Epitope Pathology and Forensic Medicine Epitopes Antigen Complementary DNA medicine Humans Immunology and Allergy Antigens Peptide sequence Autoantibodies Base Sequence Middle Aged medicine.disease Thyroid Diseases Molecular biology biology.protein Female Antibody |
| Zdroj: | Clinical Immunology and Immunopathology. 77:193-200 |
| ISSN: | 0090-1229 |
| Popis: | Random fragments of full-length 1D cDNA encoding the 64-kDa antigen were cloned and expressed in Escherichia coli as fusion proteins. Antigenic peptides were detected by colony blotting with sera from patients with thyroid-associated ophthalmopathy (TAO) and corresponding cDNA inserts were sequenced. Four antigenic peptides were characterized and positioned with respect to the 1D amino acid sequence. One antigenic peptide (G-8) was found to be close to the C-terminal end of 1D, and three others (G-2, G-4, and G-5) were clustered at sites between amino acids 76 and 205. The immunoreactivities of a panel of sera were tested against full-length 1D and the four peptides. A panel consisting of sera from (1) 15 patients with TAO, (2) 9 patients with Graves' hyperthyroidism without ophthalmopathy, (3) 6 patients with Hashimoto's thyroiditis without ophthalmopathy, and (4) 12 normal subjects were tested against the four peptides and full-length 1D protein by Western blotting. Sera from patients with TAO also reacted more often with G-4 and/or G-5 peptides than did those from normal subjects [7 of 15 (47%) sera from TAO patients versus 1 of 12 (8%) sera from normal subjects; P0.05]. Based on positioning of peptides onto the full-length 1D sequence and the reaction pattern of the serum panel, six immunogenic epitopes were identified on the 1D molecule. |
| Databáze: | OpenAIRE |
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