Molecular modeling and mechanism of action of human decay-accelerating factor
| Autor: | L. Kuttner-Kondo, Medof Me, Brodbeck W, Menachem Shoham |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Models
Molecular medicine.medical_specialty Molecular model Protein Conformation Molecular Sequence Data Bioengineering Sequence alignment Complement C3-C5 Convertases Plasma protein binding behavioral disciplines and activities Biochemistry Protein structure Internal medicine medicine Humans Amino Acid Sequence Molecular Biology Peptide sequence Decay-accelerating factor Repetitive Sequences Nucleic Acid CD55 Antigens Sequence Homology Amino Acid Chemistry C3-convertase Endocrinology Biophysics Sequence Alignment Biotechnology Protein Binding |
| Zdroj: | Protein engineering. 9(12) |
| ISSN: | 0269-2139 |
| Popis: | A model of the regulatory region of human decay accelerating factor (DAF) was built based on the known coordinates of a fragment of the structurally and functionally homologous serum protein, factor H. According to this model, the four short consensus repeats (SCRs) in DAF are arranged in a helical fashion. A positively charged surface area on SCRs 2 and 3, two of the three repeating units essential for function, is postulated to be the primary recognition site for the C3 convertases C4b2a and C3bBb. This area encompasses a cavity on SCR 2, as well as part of the groove on the SCR 2-SCR 3 interface. Two additional surface depressions are centered around the C-terminal disulfide bridges of SCRs 3 and 4. These are likely to provide additional ligand binding sites. Based on this model in conjunction with sequence homology to the Ba fragment of factor B, a mechanism of DAF's accelerated convertase decay action is postulated. |
| Databáze: | OpenAIRE |
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