Identification of Novel Thermosensors in Gram-Positive Pathogens
| Autor: | Daniela Albanesi, Diego de Mendoza, Alejandra Raquel Diaz, Pilar Fernández, María Florencia Ré, Lucía Porrini, María C. Mansilla |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology two component system ATP-binding cassette transporter Bacillus subtilis Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry 03 medical and health sciences Molecular Biosciences signalling Molecular Biology Gene lcsh:QH301-705.5 Original Research biology thermosensor biology.organism_classification gram positive pathogen Two-component regulatory system Cell biology Bacillus anthracis Response regulator 030104 developmental biology Membrane protein lcsh:Biology (General) ABC transporter Bacteria |
| Zdroj: | Frontiers in Molecular Biosciences Frontiers in Molecular Biosciences, Vol 7 (2020) |
| ISSN: | 2296-889X |
| Popis: | Temperature is a crucial variable that every living organism, from bacteria to humans, need to sense and respond to in order to adapt and survive. In particular, pathogenic bacteria exploit host-temperature sensing as a cue for triggering virulence gene expression. Here, we have identified and characterized two integral membrane thermosensor histidine kinases (HKs) from Gram-positive pathogens that exhibit high similarity to DesK, the extensively characterized cold sensor histidine kinase from Bacillus subtilis. Through in vivo experiments, we demonstrate that SA1313 from Staphylococcus aureus and BA5598 from Bacillus anthracis, which likely control the expression of putative ATP binding cassette (ABC) transporters, are regulated by environmental temperature. We show here that these HKs can phosphorylate the non-cognate response regulator DesR, partner of DesK, both in vitro and in vivo, inducing in B. subtilis the expression of the des gene upon a cold shock. In addition, we report the characterization of another DesK homolog from B. subtilis, YvfT, also closely associated to an ABC transporter. Although YvfT phosphorylates DesR in vitro, this sensor kinase can only induce des expression in B. subtilis when overexpressed together with its cognate response regulator YvfU. This finding evidences a physiological mechanism to avoid cross talk with DesK after a temperature downshift. Finally, we present data suggesting that the HKs studied in this work appear to monitor different ranges of membrane lipid properties variations to mount adaptive responses upon cooling. Overall, our findings point out that bacteria have evolved sophisticated mechanisms to assure specificity in the response to environmental stimuli. These findings pave the way to understand thermosensing mediated by membrane proteins that could have important roles upon host invasion by bacterial pathogens. |
| Databáze: | OpenAIRE |
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