Application of CP-MAS and liquid-like solid-state NMR experiments for the study of the ripening-associated cell wall changes in tomato
| Autor: | Corinne Rondeau-Mouro, Marc Lahaye, Marie-Jeanne Crépeau |
|---|---|
| Přispěvatelé: | PhysicoChimie des Macromolécules (LPCM), Institut National de la Recherche Agronomique (INRA), Laboratoire de biochimie et technologie des glucides, ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
0106 biological sciences
Magnetic Resonance Spectroscopy Time Factors Proton [SDV]Life Sciences [q-bio] Analytical chemistry 01 natural sciences Biochemistry Mass Spectrometry Cell wall 03 medical and health sciences Nuclear magnetic resonance Solanum lycopersicum Cell Wall Structural Biology Magic angle spinning SPECTROSCOPIE RMN DU SOLIDE Molecular Biology Softening Chromatography High Pressure Liquid ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Chemistry Relaxation (NMR) Temperature Ripening General Medicine Carbon [SDV] Life Sciences [q-bio] Solid-state nuclear magnetic resonance Proton NMR Protons 010606 plant biology & botany |
| Zdroj: | International Journal of Biological Macromolecules International Journal of Biological Macromolecules, Elsevier, 2003, 31, pp.235-244 |
| ISSN: | 0141-8130 |
| Popis: | 13C and 1H NMR spectra of an ethanol insoluble material (EIM) prepared from the pericarp of mature-green (MG) and red-ripe (RR) tomato fruits were acquired in 'liquid-like' and cross-polarisation with dipolar decoupling and magic angle spinning (CPMAS) conditions using the same triple resonance probe. Such a strategy allowed acquisitions of various NMR experiments aimed at detecting compositional differences as well as distinguishing differences in molecular mobility for various constituent polysaccharides related with the two ripening stages. Increase of the proton dipolar decoupling power levels from 3 to 50-55 kHz during single pulse 13C acquisition, led to more intense signals for pectic and hemicellulosic polysaccharides. This behaviour was interpreted as reflecting motional restrictions of these polysaccharides inside the porous cell wall network. Measurements of the proton rotating frame relaxation times T(1rho) in the 'liquid-like' conditions and of the proton transverse relaxation times T(2) from CPMAS spectra, revealed changes in mobilities for some pectic polysaccharides in relation with ripening, particularly for the H1 and H5 protons of alpha-1,5 arabinan (Ara) side chains of rhamnogalacturonans. These data are discussed in relation with known pectic modifications occurring during ripening and associated with the tomato fruit softening. |
| Databáze: | OpenAIRE |
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