Isolation and Characterization of a Functional Murine Interferon Alpha Gene which Is Not Expressed in Fibroblasts upon Virus Induction
| Autor: | M. Dion, Sébastien Navarro, J. Doly, F. Berlot-Picard, G. Vodjdani |
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| Rok vydání: | 1989 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Restriction Mapping Newcastle disease virus Alpha interferon Regulatory Sequences Nucleic Acid Biology Vesicular stomatitis Indiana virus Homology (biology) Cell Line Mice Interferon Sequence Homology Nucleic Acid Virology Gene expression medicine Animals Gene family Gene Base Sequence Nucleic acid sequence Fibroblasts Cosmids Molecular biology Poly I-C Gene Expression Regulation Genes Protein Biosynthesis Interferon Type I Cosmid RNA medicine.drug |
| Zdroj: | Journal of General Virology. 70:1381-1389 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-70-6-1381 |
| Popis: | A mouse genomic segment containing three new members of the murine interferon alpha (MuIFN-alpha) gene family was isolated from a fibroblastic cosmid library. A 4 kb EcoRI fragment contained a new MuIFN-alpha gene named MuIFN-alpha 8. The nucleotide sequence of the coding and flanking regions of this gene showed a high level of homology to those of known members of the MuIFN-alpha family. Transient expression of the MuIFN-alpha 8 gene in COS cells and oocyte translation of in vitro transcripts both led to a biologically active protein. The antiviral activity was neutralized by monoclonal and polyclonal MuIFN-alpha antibodies. Although the 5' flanking sequence shows features characteristic of an IFN regulatory region, the MuIFN-alpha 8 gene is not expressed in murine fibroblasts treated with Newcastle disease virus or poly(I).poly(C). |
| Databáze: | OpenAIRE |
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