Fermentation product butane 2,3-diol induces Ca2+ transients in E. coli through activation of lanthanum-sensitive Ca2+ channels
| Autor: | Kenneth Taylor Wann, Anthony K. Campbell, Riffat Naseem, Stephanie Beatrix Matthews, I. Barry Holland |
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| Přispěvatelé: | Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2007 |
| Předmět: |
inorganic chemicals
Physiology Stereochemistry MESH: Lanthanum Diol chemistry.chemical_element Calcium MESH: Calcium Signaling MESH: Fermentation MESH: Butylene Glycols MESH: Dose-Response Relationship Drug MESH: Propane Propane 03 medical and health sciences chemistry.chemical_compound Cytosol MESH: Cytosol Lanthanum Escherichia coli polycyclic compounds [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Calcium Signaling Butylene Glycols Molecular Biology Ion channel 030304 developmental biology 0303 health sciences Dose-Response Relationship Drug integumentary system Voltage-dependent calcium channel MESH: Escherichia coli 030306 microbiology organic chemicals Stereoisomerism Butane Cell Biology MESH: Stereoisomerism chemistry MESH: Calcium Fermentation MESH: Calcium Channels Calcium Channels Ethylene glycol hormones hormone substitutes and hormone antagonists |
| Zdroj: | Cell Calcium Cell Calcium, Elsevier, 2007, 41 (2), pp.97-106. ⟨10.1016/j.ceca.2006.05.007⟩ |
| ISSN: | 0143-4160 |
| DOI: | 10.1016/j.ceca.2006.05.007 |
| Popis: | The results here are the first demonstration of a physiological agonist opening Ca2+ channels in bacteria. Bacteria in the gut ferment glucose and other substrates, producing alcohols, diols, ketones and acids, that play a key role in lactose intolerance, through the activation of Ca2+ and other ion channels in host cells and neighbouring bacteria. Here we show butane 2,3-diol (5–200 mM; half maximum 25 mM) activates Ca2+ transients in E. coli, monitored by aequorin. Ca2+-transient magnitude depended on external Ca2+ (0.1–10 mM). meso-Butane 2,3-diol was approximately twice as potent as 2R,3R (?) and 2S,3S (+) butane 2,3-diol. There were no detectable effects on cytosolic free Ca2+ of butane 1,3-diol, butane 1,4-diol and ethylene glycol. The glycerol fermentation product propane 1,3-diol only induced significant Ca2+ transients in 10 mM external Ca2. Ca2+ butane 2,3-diol Ca2+ transients were due to activation of Ca2+ influx, followed by activation of Ca2+ efflux. The effect of butane 2,3-diol was abolished by La3+, and markedly reduced as a function of growth phase. These results were consistent with butane 2,3-diol activating a novel La3+-sensitive Ca2+ channel. They have important implications for the role of butane 2,3-diol and Ca2+ in bacterial-host cell signalling. |
| Databáze: | OpenAIRE |
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