CHF: a novel factor binding to cyclin A CHR corepressor element
| Autor: | Annick Vié, Alexandre Philips, Ned J.C. Lamb, Séverine Chambeyron, Jean Marie Blanchard |
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| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
Microinjections Transcription Genetic Macromolecular Substances T-Lymphocytes Molecular Sequence Data Cyclin A Repressor Regulatory Sequences Nucleic Acid Biology Binding Competitive Resting Phase Cell Cycle S Phase Mice Genetics Animals Humans Hydroxyurea Binding site Promoter Regions Genetic E2F Molecular Biology Cyclin-dependent kinase 1 Binding Sites Base Sequence Cell Cycle Cell cycle Cell Cycle Gene Cell biology Molecular Weight Chromatography Gel biology.protein Electrophoresis Polyacrylamide Gel Corepressor Transcription Factors |
| Zdroj: | Oncogene. 18:6222-6232 |
| ISSN: | 1476-5594 0950-9232 |
| Popis: | Cell cycle modulation of cyclin A expression is due to the periodic relief of a transcriptional repression mediated by a bipartite negative DNA regulatory region. The 5' element (Cell Cycle Responsive Element: CCRE; cell Cycle Dependent Element: CDE) is clearly occupied in a cyclic manner in vivo, whereas the 3' element, whose sequence is shared by B-myb, cdc25C and cdc2 genes (cell Cycle gene Homology Region: CHR), is involved in more subtle interactions. Mutation of either element results in complete deregulation of cyclin A promoter activity. Whereas some reports claim that E2F/DP can bind to the CCRE/CDE, the nature of the protein(s) interacting with the CHR is unknown. In the present work we have characterized an activity present in quiescent cells and absent in cells blocked in S phase, which binds specifically to cyclin A CHR, but not to B-myb, or to cdc25C, or to cdc2 CHRs. A 90 kD protein, named CHF (cyclin A CHR binding factor), has been identified through preparative electrophoresis and UV crosslinking experiments. In order to address in more functional terms the binding of CHF to cyclin A CHR, we developed in vitro and in vivo oligonucleotide competition assays. Both in vitro transcription and in vivo microinjection experiments demonstrate that a functional difference exists between the composite CCRE/CDE-CHR repressor regions of cell cycle regulated genes such as cyclin A and cdc25C. |
| Databáze: | OpenAIRE |
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