NAD-dependent hydrogenase from Alcaligenes eutrophus Z1: Does it have a regulatory centre?
| Autor: | Iliya B. Utkin, Vladimir Popov, Alexey M. Egorov, Iliya V. Berezin, Rem R. Petrov |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
chemistry.chemical_classification
Hydrogenase biology Chemistry Electron Spin Resonance Spectroscopy Biophysics Nad dependent Cell Biology Alcaligenes eutrophus NAD biology.organism_classification Photochemistry Biochemistry Redox Enzyme Activation Enzyme activator Enzyme Oxidizing agent Alcaligenes Oxidation-Reduction Molecular Biology Bacteria |
| Zdroj: | Biochemical and Biophysical Research Communications. 142:297-301 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(87)90272-5 |
| Popis: | Evidence is presented for the existence of a relatively high-potential regulatory centre in the NAD-dependent hydrogenase from the hydrogen oxidizing bacterium Alcaligenes eutrophus Z1. Reduction of the hydrogenase to the redox potentials lower than -100 mV converts the enzyme into a catalytically active state that is remarkably stable to oxidants. Once activated, the enzyme does not loose its activity on intensive oxygenation for at least 3 hours. A novel hydrogenase ESR signal with a wide temperature optimum and a approximately -100 mV midpoint redox potential was detected. We suggest that the reduction of this redox centre trigger conformational changes in the inactive oxidized enzyme molecule, thus reorganizing the latter into the active one. |
| Databáze: | OpenAIRE |
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