Construction of a thiamin sensor from the periplasmic thiamin binding protein
| Autor: | Erika V. Soriano, Tadhg P. Begley, Steven E. Ealick, Jeremiah Hanes, Debashree Chatterjee |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Biosensing Techniques
Article Catalysis Thiamin binding chemistry.chemical_compound Coumarins Materials Chemistry Thiamine Metals and Alloys Amino acid substitution General Chemistry Periplasmic space Coumarin Fluorescence Protein Structure Tertiary Surfaces Coatings and Films Electronic Optical and Magnetic Materials Triple mutant Kinetics Amino Acid Substitution Biochemistry chemistry Carrier protein Mutation Periplasm Ceramics and Composites Carrier Proteins |
| Zdroj: | Chem. Commun.. 47:2273-2275 |
| ISSN: | 1364-548X 1359-7345 |
| DOI: | 10.1039/c0cc04228e |
| Popis: | This communication describes the development of a thiamin sensor based on the bacterial thiamin binding protein. A triple mutant (C48S, C50S, S62C) of TbpA was labeled on C62 with N-[2-(L-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC). Thiamin binding to this protein reduced the coumarin fluorescence giving a thiamin sensor with low nanomolar sensitivity. |
| Databáze: | OpenAIRE |
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