Additional file 3 of Development of a new barcode-based, multiplex-PCR, next-generation-sequencing assay and data processing and analytical pipeline for multiplicity of infection detection of Plasmodium falciparum

Autor: Mitchell, Rebecca M., Zhiyong Zhou, Sheth, Mili, Sergent, Sheila, Frace, Michael, Vishal Nayak, Hu, Bin, Gimnig, John, Kuile, Feiko Ter, Lindblade, Kim, Slutsker, Laurence, Hamel, Mary J., Meghna Desai, Kephas Otieno, Kariuki, Simon, Ymir Vigfusson, Shi, Ya Ping
Rok vydání: 2021
DOI: 10.6084/m9.figshare.14052756.v1
Popis: Additional file 3: Figure S3. Plot SNP23 from Combination A mixes. Plot SNP23 from Combination A mixes was made separately from all other SNPs. Data is also separated by source material (DBS and frozen blood samples). Within each mixture, left to right, the colors are: blue—all strains identical, orange—dominant strain unique, green—intermediate strain unique, yellow—low strain unique, and gray—SNP23 in Combination A mixes. A pilot experiment was also conducted using a different culture source, and this difference at SNP23 was not observed. Based on the consistency among all other SNP sites and the difference between parasite batches, SNP23 from Combination A was excluded from the Fig. 3 analysis concerning two distinct SNP reads.
Databáze: OpenAIRE