Biocatalytically induced surface modification of the tobacco mosaic virus and the bacteriophage M13
| Autor: | Rainer Fischer, Bas-Jan Hoogenberg, Irene Lodoso-Torrecilla, Eliza M. Warszawik, Cathelijn A. J. van Nisselroy, Sybren Dantuma, Frank Hollmann, Valentina Vignali, Barbara Santos de Miranda, Patrick van Rijn, Ulrich Commandeur, Jan Willem de Vries |
|---|---|
| Přispěvatelé: | Polymer Chemistry and Bioengineering, Nanotechnology and Biophysics in Medicine (NANOBIOMED), Restoring Organ Function by Means of Regenerative Medicine (REGENERATE) |
| Rok vydání: | 2018 |
| Předmět: |
viruses
010402 general chemistry 01 natural sciences Catalysis Bacteriophage chemistry.chemical_compound Capsid Microscopy Electron Transmission Materials Chemistry Tobacco mosaic virus PARTICLES Tyrosine Free Radical Formation Acrylate biology 010405 organic chemistry Laccase Metals and Alloys General Chemistry biology.organism_classification Combinatorial chemistry 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Tobacco Mosaic Virus POLYMERIZATION Monomer chemistry Acrylates Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Ceramics and Composites Biocatalysis Surface modification Oxidation-Reduction Bacteriophage M13 |
| Zdroj: | Chemical communications (Cambridge, England), 55(1), 51-54. ROYAL SOC CHEMISTRY Chemical Communications, 55(1) Chemical Communications |
| ISSN: | 1364-548X 1359-7345 |
| Popis: | Engineered viruses are finding an increasing number of applications in basic, translational research and materials science. Genetic and chemical engineering of capsids represents a key point for tailoring the properties of viral particles, but the synthetic efforts and limits accompanying these processes still hinder their usability. Here, a single-step highly selective biocatalytic functionalization approach is described, providing a general platform for virus-acrylate hybrid particles. The tobacco mosaic virus (TMV) and the bacteriophage M13 have been successfully modified via laccase induced free radical formation on the tyrosine residues through single electron oxidation as the initiating step and the free radicals subsequently react with acrylate-based monomers. This new approach can be extended to other biomolecular assemblies with surface exposed tyrosine residues, when the introduction of new functionalities is desired. |
| Databáze: | OpenAIRE |
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