Transfer of a grapevine stilbene synthase gene to rice (Oryza sativa L.)

Autor:	P. Stark-Lorenzen, J. E. Thomzik, G. Hänßler, H.-P. Mühlbach, B. Nelke
Rok vydání:	2019
Předmět:	Pyricularia Oryza sativa biology fungi Genetic transfer food and beverages Kanamycin Plant Science General Medicine Genetically modified crops biology.organism_classification Genetically modified rice Transformation (genetics) Botany medicine Agronomy and Crop Science Gene medicine.drug
Zdroj:	Plant cell reports. 16(10)
ISSN:	1432-203X
Popis:	A gene derived from grapevine (Vitis vinifera) coding for stilbene synthase has been transferred into protoplasts of the commercially important japonica rice cultivar Nipponbare using PEG-mediated direct gene transfer. Transgenic plants were regenerated from calli selected on kanamycin. Southern blot analysis of genomic DNA isolated from regenerants and progeny plants demonstrated that the stilbene synthase gene is stably integrated in the genome of transgenic rice plants and inherited in the offspring. The transient formation of stilbene-synthase-specific mRNA shortly after inoculation with the fungus of the rice blast Pyricularia oryzae has demonstrated that the grapevine stilbene synthase promoter is also active in monocotyledonous plants. Preliminary results indicate an enhanced resistance of transgenic rice to P. oryzae.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42f745781de8109318f8179d7e0030fd https://pubmed.ncbi.nlm.nih.gov/30727616 Zobrazit plný text záznamu Full text from SpringerLink