A vaccine against S. pyogenes: Design and experimental immune response
| Autor: | Oliver Pérez, S. Freschi de Barros, Caridad Zayas, William Ribeiro da Silva, Jorge Kalil, R. Alencar, Edilberto Postol, C. R. Puschel, Miriam Lastre, Luiza Guilherme, F. Higa, Vanessa de Moura Sá-Rocha, Luiz Carlos de Sá-Rocha |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Meningitides
Streptococcus pyogenes medicine.medical_treatment Molecular Sequence Data medicine.disease_cause General Biochemistry Genetics and Molecular Biology Epitope Mice Immune system Immunity medicine Animals Amino Acid Sequence Immunity Mucosal Molecular Biology IMUNIDADE Mice Inbred BALB C biology Streptococcal Vaccines biology.organism_classification Virology Drug Design Immunology biology.protein Female Neisseria Antibody Adjuvant |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/j.ymeth.2009.03.024 |
| Popis: | Streptococcus pyogenes causes severe invasive infections: the post-streptococcal sequelae of acute rheumatic fever (RF) and rheumatic heart disease (RHD), acute glomerulonephritis, and uncomplicated pharyngitis and pyoderma. Efforts to produce a vaccine against S. pyogenes began several decades ago, and different models have been proposed. Here, we describe the methodology used in the development of a new vaccine model, consisting of both T and B protective epitopes constructed as synthetic peptides and recombinant proteins. Two adjuvants were tested in an experimental inbred mouse model: a classical Freund's adjuvant and a new adjuvant (AFCo1) that induces mucosal immune responses and is obtained by calcium precipitation of a proteoliposome derived from the outer membrane of Neisseria meningitides B. The StreptInCor vaccine epitope co-administrated with AFCo1 adjuvant induced mucosal (IgA) and systemic (IgG) antibodies as preferential Th1-mediated immune responses. No autoimmune reactions were observed, suggesting that the vaccine epitope is safe. |
| Databáze: | OpenAIRE |
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