Nanostructural Diversity of Synapses in the Mammalian Spinal Cord
| Autor: | Frances Elizabeth Goff, Gareth B. Miles, Jonathan Nylk, Kishan Dholakia, Calum Bonthron, Fei Zhu, Sumi Bez, Matthew J. Broadhead, Frank J. Gunn-Moore, Lauren Arcinas, Seth G. N. Grant |
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| Přispěvatelé: | BBSRC, Chief Scientist Office, University of St Andrews. School of Psychology and Neuroscience, University of St Andrews. School of Biology, University of St Andrews. School of Physics and Astronomy, University of St Andrews. Centre for Biophotonics, University of St Andrews. Institute of Behavioural and Neural Sciences |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
NDAS lcsh:Medicine Biology Neural circuits Article 03 medical and health sciences 0302 clinical medicine Age groups Postsynaptic potential medicine Biological neural network lcsh:Science Developmental stage Spine regulation and structure Multidisciplinary lcsh:R Spinal cord Lumbar Spinal Cord 030104 developmental biology medicine.anatomical_structure RC0321 Excitatory postsynaptic potential lcsh:Q Astrocyte RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 10, Iss 1, Pp 1-18 (2020) Broadhead, M J, Bonthron, C, Arcinas, L, Bez, S, Zhu, F, Goff, F, Nylk, J, Dholakia, K, Gunn-moore, F, Grant, S G N & Miles, G B 2020, ' Nanostructural Diversity of Synapses in the Mammalian Spinal Cord ', Scientific Reports, vol. 10, no. 1 . https://doi.org/10.1038/s41598-020-64874-9 |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-64874-9 |
| Popis: | This work for funded by the Biotechnology and Biological Sciences Research Council (BBSRC; BB/M021793/1), RS MacDonald Charitable Trust, Motor Neurone Disease (MND) Association UK (Miles/Apr18/863-791), the Engineering and Physical Sciences Research Council (EPSRC; EP/P030017/1), Welcome Trust (202932/Z/16/Z), European Research Council (ERC; 695568) and the Simons Initiative for the Developing Brain. Functionally distinct synapses exhibit diverse and complex organisation at molecular and nanoscale levels. Synaptic diversity may be dependent on developmental stage, anatomical locus and the neural circuit within which synapses reside. Furthermore, astrocytes, which align with pre and post-synaptic structures to form “tripartite synapses”, can modulate neural circuits and impact on synaptic organisation. In this study, we aimed to determine which factors impact the diversity of excitatory synapses throughout the lumbar spinal cord. We used PSD95-eGFP mice, to visualise excitatory postsynaptic densities (PSDs) using high-resolution and super-resolution microscopy. We reveal a detailed and quantitative map of the features of excitatory synapses in the lumbar spinal cord, detailing synaptic diversity that is dependent on developmental stage, anatomical region and whether associated with VGLUT1 or VGLUT2 terminals. We report that PSDs are nanostructurally distinct between spinal laminae and across age groups. PSDs receiving VGLUT1 inputs also show enhanced nanostructural complexity compared with those receiving VGLUT2 inputs, suggesting pathway-specific diversity. Finally, we show that PSDs exhibit greater nanostructural complexity when part of tripartite synapses, and we provide evidence that astrocytic activation enhances PSD95 expression. Taken together, these results provide novel insights into the regulation and diversification of synapses across functionally distinct spinal regions and advance our general understanding of the ‘rules’ governing synaptic nanostructural organisation. Publisher PDF |
| Databáze: | OpenAIRE |
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