A Single Immunization With Modified Vaccinia Virus Ankara-Based Influenza Virus H7 Vaccine Affords Protection in the Influenza A(H7N9) Pneumonia Ferret Model
| Autor: | Geert van Amerongen, Stella E. Vogelzang-van Trierum, Lidewij Wiersma, Joost H. C. M. Kreijtz, Albert D. M. E. Osterhaus, Heidi L. M. De Gruyter, Guus F. Rimmelzwaan, Ron A. M. Fouchier, Koert J. Stittelaar, Gerd Sutter |
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| Přispěvatelé: | Virology |
| Rok vydání: | 2014 |
| Předmět: |
viruses
Genetic Vectors Orthomyxoviridae Hemagglutinin (influenza) Hemagglutinin Glycoproteins Influenza Virus Vaccinia virus Biology Influenza A Virus H7N9 Subtype medicine.disease_cause Virus Microbiology Major Articles and Brief Reports chemistry.chemical_compound Orthomyxoviridae Infections SDG 3 - Good Health and Well-being Influenza A virus medicine Animals Immunology and Allergy Drug Carriers Vaccination Ferrets biology.organism_classification Virology Influenza A virus subtype H5N1 Disease Models Animal Treatment Outcome Infectious Diseases Immunization chemistry Influenza Vaccines biology.protein Female Vaccinia Lung Diseases Interstitial |
| Zdroj: | Journal of Infectious Diseases, 211(5), 791-800. Oxford University Press |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Since the first reports in early 2013, >440 human cases of infection with avian influenza A(H7N9) have been reported including 122 fatalities. After the isolation of the first A(H7N9) viruses, the nucleotide sequences became publically available. Based on the coding sequence of the influenza virus A/Shanghai/2/2013 hemagglutinin gene, a codon-optimized gene was synthesized and cloned into a recombinant modified vaccinia virus Ankara (MVA). This MVA-H7-Sh2 viral vector was used to immunize ferrets and proved to be immunogenic, even after a single immunization. Subsequently, ferrets were challenged with influenza virus A/Anhui/1/2013 via the intratracheal route. Unprotected animals that were mock vaccinated or received empty vector developed interstitial pneumonia characterized by a marked alveolitis, accompanied by loss of appetite, weight loss, and heavy breathing. In contrast, animals vaccinated with MVA-H7-Sh2 were protected from severe disease. |
| Databáze: | OpenAIRE |
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