Tissue-engineered human psoriatic skin supplemented with cytokines as an in vitro model to study plaque psoriasis
| Autor: | Roxane Pouliot, Sylvain L. Guérin, Claudia Pouliot-Bérubé, Karine Zaniolo |
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| Rok vydání: | 2016 |
| Předmět: |
Keratinocytes
Male 0301 basic medicine Embryology medicine.medical_treatment Biomedical Engineering In Vitro Techniques Biology Transcriptome 03 medical and health sciences Psoriatic skin Psoriasis Gene expression medicine Humans Gene Cells Cultured Skin Skin Artificial Tissue engineered Tissue Engineering Gene Expression Profiling Skin Transplantation Fibroblasts Middle Aged medicine.disease 030104 developmental biology Cytokine Case-Control Studies Immunology Cytokines Female DNA microarray Biomarkers |
| Zdroj: | Regenerative Medicine. 11:545-557 |
| ISSN: | 1746-076X 1746-0751 |
| DOI: | 10.2217/rme-2016-0037 |
| Popis: | Aim: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used. Materials & methods: Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-α, IL-1α, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray. Results: Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU). The expression of those genes was dramatically increased compared with basal levels of controls (p |
| Databáze: | OpenAIRE |
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