Genomic analysis of Grapevine Retrotransposon 1 (Gret1) in Vitis vinifera
| Autor: | H. Sofia Pereira, Leonor Morais-Cecílio, Augusta Barão, Wanda Viegas, Margarida Delgado |
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| Rok vydání: | 2005 |
| Předmět: |
Retroelements
Euchromatin Restriction Mapping Retrotransposon Biology Plant Roots Genome Complete sequence Genetics Vitis Cultivar Vitis vinifera Interphase In Situ Hybridization Fluorescence Metaphase DNA Primers Regulation of gene expression Base Sequence fungi Chromosome Mapping food and beverages General Medicine Phenotype Agronomy and Crop Science Genome Plant Biotechnology |
| Zdroj: | Theoretical and Applied Genetics. 111:871-878 |
| ISSN: | 1432-2242 0040-5752 |
| DOI: | 10.1007/s00122-005-0009-0 |
| Popis: | The complete sequence of the first retrotransposon isolated in Vitis vinifera, Gret 1, was used to design primers that permitted its analysis in the genome of grapevine cultivars. This retroelement was found to be dispersed throughout the genome with sites of repeated insertions. Fluorescent in situ hybridization indicated multiple Gret 1 loci distributed throughout euchromatic portions of chromosomes. REMAP and IRAP proved to be useful as molecular markers in grapevine. Both of these techniques showed polymorphisms between cultivars but not between clones of the same cultivar, indicating differences in Gret 1 distribution between cultivars. The combined cytological and molecular results suggest that Gret 1 may have a role in gene regulation and in explaining the enormous phenotypic variability that exists between cultivars. |
| Databáze: | OpenAIRE |
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