YraP Contributes to Cell Envelope Integrity and Virulence of Salmonella enterica Serovar Typhimurium
| Autor: | Riyaz Maderbocus, Timothy J. Wells, Amanda E. Rossiter, Jessica L. Rooke, Adam F. Cunningham, Georgia L. Isom, Jennifer L. Marshall, Yanina R. Sevastsyanovich, Jack A. Bryant, Anna E. Schager, Ian R. Henderson, Timothy J. Knowles, Derrick J. P. Squire, Faye C. Morris, Michael Overduin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Salmonella typhimurium Salmonella Lipopolysaccharide Virulence Factors Lipoproteins 030106 microbiology Immunology Virulence Microbial Sensitivity Tests medicine.disease_cause Microbiology Cell Line 03 medical and health sciences chemistry.chemical_compound medicine Animals Humans Escherichia coli biology Neisseria meningitidis Macrophages Epithelial Cells Bacterial Infections biology.organism_classification Anti-Bacterial Agents Mice Inbred C57BL Disease Models Animal 030104 developmental biology Infectious Diseases chemistry Salmonella enterica Mutation Salmonella Infections Parasitology Cell envelope Bacterial outer membrane Bacterial Outer Membrane Proteins |
| Zdroj: | Infection and immunity. 86(11) |
| ISSN: | 1098-5522 |
| Popis: | Mutations in σ(E)-regulated lipoproteins have previously been shown to impact bacterial viability under conditions of stress and during in vivo infection. YraP is conserved across a number of Gram-negative pathogens, including Neisseria meningitidis, where the homolog is a component of the Bexsero meningococcal group B vaccine. Investigations using laboratory-adapted Escherichia coli K-12 have shown that yraP mutants have elevated sensitivity to a range of compounds, including detergents and normally ineffective antibiotics. In this study, we investigate the role of the outer membrane lipoprotein YraP in the pathogenesis of Salmonella enterica serovar Typhimurium. We show that mutations in S. Typhimurium yraP result in a defective outer membrane barrier with elevated sensitivity to a range of compounds. This defect is associated with attenuated virulence in an oral infection model and during the early stages of systemic infection. We show that this attenuation is not a result of defects in lipopolysaccharide and O-antigen synthesis, changes in outer membrane protein levels, or the ability to adhere to and invade eukaryotic cell lines in vitro. |
| Databáze: | OpenAIRE |
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