Polyclonal antibody–based immunohistochemical detection of intraleukocytic Theileria parasites in roan and sable antelopes
| Autor: | Katja Natalie Koeppel, Jeanni Fehrsen, Bernat Martí-Garcia, Emily P. Mitchell, Ilse Vorster, Sarah J. Clift, Antoinette I. Josemans, Rephima M. Phaswane |
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| Rok vydání: | 2021 |
| Předmět: |
040301 veterinary sciences
Cattle Diseases Cryptosporidiosis Cryptosporidium Biology 0403 veterinary science 03 medical and health sciences Theileria parasitic diseases Mustelidae Animals Parasites Full Scientific Reports 030304 developmental biology 0303 health sciences Theileria sp General Veterinary Immunoperoxidase 04 agricultural and veterinary sciences Hippotragus equinus biology.organism_classification Virology Theileriasis Antelopes Polyclonal antibodies biology.protein Immunohistochemistry Cattle |
| Zdroj: | J Vet Diagn Invest |
| ISSN: | 1943-4936 1040-6387 |
| DOI: | 10.1177/10406387211033272 |
| Popis: | Theileria parasites commonly infect African wild artiodactyls. In rare roan ( Hippotragus equinus) and sable ( H. niger) antelopes, Theileria sp. (sable)-associated calf mortalities constrain breeding programs. The pathogenicity of most leukocyte-transforming Theileria spp. originates in their invasion of and multiplication in various mononuclear leukocytes, the transformation of both infected and uninfected leukocytes, and their infiltration of multiple organs. Understanding the pathogenesis of theileriosis can be improved by the use of immunohistochemistry (IHC) to identify the localization of the parasites in tissue sections. Our aim was to develop a reproducible IHC assay to detect leukocyte-associated Theileria parasites in formalin-fixed, paraffin-embedded roan and sable tissues. Polyclonal antibodies were purified from the sera of 5 roans from an area endemic for Theileria sp. (sable) and tested for IHC reactivity in 55 infected and 39 control roan and sable antelopes, and for antigen and species cross-reactivity in an additional 58 cases. The 3 strongest antibodies consistently detected intraleukocytic theilerial antigens in known positive cases in roan and sable antelopes, and also detected other Theileria spp. in non-hippotraginid wild artiodactyl tissues. The antibodies did not cross-react with other apicomplexan protozoa, with the exception of Cryptosporidium. Given that PCR on its own cannot determine the significance of theilerial infection in wild ruminants, IHC is a useful laboratory test with which to confirm the diagnosis in these species. |
| Databáze: | OpenAIRE |
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