Anti-tumor and immunomodulatory activities induced by an alkali-extracted polysaccharide BCAP-1 from Bupleurum chinense via NF-κB signaling pathway
| Autor: | Tiancheng Lu, Xiangfu Song, Ting Ren, Zhou Zheng, Zhicheng Wang, Haibin Tong, Fengguo Du |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Antineoplastic Agents 02 engineering and technology Alkalies Polysaccharide Plant Roots Biochemistry Immunomodulation Sepharose Mice 03 medical and health sciences Polysaccharides Structural Biology Cell Line Tumor Animals MTT assay Secretion Cytotoxicity Molecular Biology chemistry.chemical_classification biology NF-kappa B General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Molecular biology Bupleurum 030104 developmental biology chemistry Bupleurum chinense Phosphorylation Signal transduction 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 95:357-362 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2016.10.112 |
| Popis: | Bupleurum chinense is a well-known traditional Chinese medicine. Polysaccharides extracted from medical plants possess multiple healthy benefits. In the present study, an alkali-extracted polysaccharide (BCAP-1) was isolated from Bupleurum chinense, and evaluated its physicochemical features, anti-tumor activities and immunomodulatory effects. BCAP-1 was obtained by alkali-extraction, ethanol precipitation, and fractionation by DEAE-cellulose and Sepharose CL-6B columns. BCAP-1 markedly inhibited Sarcoma 180tumor growth in tumor-bearing mice, and increased the secretion of TNF-α in serum. MTT assay showed that BCAP-1 had no cytotoxicity against S-180 tumor cells. BCAP-1 enhanced the secretion of TNF-α and NO, and the transcripts of TNF-α and iNOS were increased. Meanwhile, BCAP-1 treatment induced the phosphorylation of p65 and decreased the expression of IκB in macrophages. These results suggest that BCAP-1 could activate macrophages through NF-κB signaling pathway, and the anti-tumor effects of BCAP-1 can be achieved by its immunostimulating features. |
| Databáze: | OpenAIRE |
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