Tomato chlorotic mottle virus is a target of RNA silencing but the presence of specific short interfering RNAs does not guarantee resistance in transgenic plants
| Autor: | Simone G. Ribeiro, Hendrikus Lohuis, Marcel Prins, Rob Goldbach |
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| Přispěvatelé: | Simone da Graça Ribeiro, Embrapa Recursos Genéticos e Biotecnologia, Hendrikus Lohuis, Wageningen University, Rob Goldbach, Wageningen University, Marcel Prins, Wageningen University. |
| Rok vydání: | 2006 |
| Předmět: |
viruses
Resistance Laboratory of Virology Nicotiana benthamiana Cellular Response to Infection Solanum lycopersicum RNA interference whitefly-transmitted geminiviruses genetic-analysis RNA Small Interfering biology food and beverages DNA virus Plants Genetically Modified Antisense RNA antisense rna Petunia RNA silencing coat protein RNA Plant Begomovirus RNA Interference dna virus replication curl-sardinia-virus Immunology Trans-acting siRNA broad-spectrum resistance Genome Viral Microbiology Tomato Laboratorium voor Virologie Viral Proteins Species Specificity Tomate Virology Tobacco Plant Diseases RNA Double-Stranded golden mosaic-virus double-stranded-rna fungi Vírus RNA biology.organism_classification Plant Leaves Insect Science DNA Viral Planta Transgênica |
| Zdroj: | Journal of Virology 81 (2007) 4 Journal of Virology, 81(4), 1563-1573 Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 0022-538X |
| Popis: | Tomato chlorotic mottle virus (ToCMoV) is a begomovirus found widespread in tomato fields in Brazil. ToCMoV isolate BA-Se1 (ToCMoV-[BA-Se1]) was shown to trigger the plant RNA silencing surveillance in different host plants and, coinciding with a decrease in viral DNA levels, small interfering RNAs (siRNAs) specific to ToCMoV-[BA-Se1] accumulated in infected plants. Although not homogeneously distributed, the siRNA population in both infected Nicotiana benthamiana and tomato plants represented the entire DNA-A and DNA-B genomes. We determined that in N. benthamiana , the primary targets corresponded to the 5′ end of AC1 and the embedded AC4, the intergenic region and 5′ end of AV1 and overlapping central part of AC5. Subsequently, transgenic N. benthamiana plants were generated that were preprogrammed to express double-stranded RNA corresponding to this most targeted portion of the virus genome by using an intron-hairpin construct. These plants were shown to indeed produce ToCMoV-specific siRNAs. When challenge inoculated, most transgenic lines showed significant delays in symptom development, and two lines had immune plants. Interestingly, the levels of transgene-produced siRNAs were similar in resistant and susceptible siblings of the same line. This indicates that, in contrast to RNA viruses, the mere presence of transgene siRNAs corresponding to DNA virus sequences does not guarantee virus resistance and that other factors may play a role in determining RNA-mediated resistance to DNA viruses. |
| Databáze: | OpenAIRE |
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