Imbalance of Th17/Tregs in rats with smoke inhalation-induced acute lung injury
| Autor: | Fan Zhang, Chengbin Wang, Mianyang Li, Ya-ting Lan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Membrane permeability Smoke inhalation Cell Acute Lung Injury Vascular permeability Lung injury T-Lymphocytes Regulatory Article Immunophenotyping Pathogenesis Capillary Permeability 03 medical and health sciences Leukocyte Count 0302 clinical medicine Smoke medicine Animals IL-2 receptor Lymphocyte Count Lung Peroxidase Multidisciplinary medicine.diagnostic_test business.industry hemic and immune systems respiratory system Smoke Inhalation Injury medicine.disease Lymphocyte Subsets respiratory tract diseases Rats 030104 developmental biology Bronchoalveolar lavage medicine.anatomical_structure Phenotype Immunology Th17 Cells business Bronchoalveolar Lavage Fluid 030215 immunology |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | T helper (Th) 17 cells and CD4+ CD25+ regulatory T (Treg) cells are supposed to be critically involved in regulating autoimmune and inflammatory diseases. The aim of this study was to investigate the Th17/Treg pattern in rats with gunpowder smog-induced acute lung injury. Wistar rats were equally randomized to three groups: normal control group, ALI 6 h group (smoke inhalation for 6 h) and ALI 24 h group (smoke inhalation for 24 h). We observed changes in cell counting in bronchoalveolar lavage fluid (BALF), alveolar-capillary membrane permeability and lung tissue pathology. Moreover, rats in ALI 6 h and ALI 24 h group showed increased expression of Th17 cell and related cytokines (IL-17 A, IL-6, TGF-β and IL-23). Meanwhile, Treg prevalence and related cytokines (IL-10, IL-2 and IL-35) were decreased. Consequently, the ratio of Th17/Treg was higher after smoke inhalation. Additionally, Th1 cell decreased while Th2 cell increased at 6 h and 24 h after smoke inhalation. In conclusion, Th17/Treg imbalance exists in rats with smoke inhalation-induced acute lung injury, suggesting its potential role in the pathogenesis of this disease. |
| Databáze: | OpenAIRE |
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