Isolation of adult mouse microglia using their in vitro adherent properties
| Autor: | Mike Dragunow, Kevin Lee, Zoe Woolf, Maurice A. Curtis, Yewon Jung, Johanna M. Montgomery, Taylor J. Stevenson, Thomas I. H. Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Aging
Science (General) Central nervous system Cell Separation Biology General Biochemistry Genetics and Molecular Biology Mice Q1-390 Fetal mouse Protocol medicine Animals Cell isolation Innate immune system General Immunology and Microbiology Microglia General Neuroscience Brain In vitro Cell biology medicine.anatomical_structure nervous system Cell culture Neuroscience |
| Zdroj: | STAR Protocols, Vol 2, Iss 2, Pp 100518-(2021) STAR Protocols |
| ISSN: | 2666-1667 |
| Popis: | Summary Microglia are the primary innate immune effectors of the central nervous system. Although numerous protocols have been developed to isolate fetal mouse microglia, the isolation of adult mouse microglia has proven more difficult. Here, we present a simple, widely accessible protocol to isolate pure microglia cultures from 4- to 14-month-old mouse brains using their adherent properties in vitro. These isolated microglia recapitulate the adherent properties of adult human microglia and present a more suitable model for studying age-related diseases. For complete details on the use and execution of this protocol in adult human microglia, please refer to Rustenhoven et al. (2016). Graphical abstract Highlights • An optimized protocol for the isolation of pure adult mouse microglia • Utilization of the adherent properties of adult mouse microglia for isolation • Isolated microglial cultures suitable for a range of functional assays Microglia are the primary innate immune effectors of the central nervous system. Although numerous protocols have been developed to isolate fetal mouse microglia, the isolation of adult mouse microglia has proven more difficult. Here, we present a simple, widely accessible protocol to isolate pure microglia cultures from 4- to 14-month-old mouse brains using their adherent properties in vitro. These isolated microglia recapitulate the adherent properties of adult human microglia and present a more suitable model for studying age-related diseases. |
| Databáze: | OpenAIRE |
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