Chromosome 6 Encoded RNaseT2 Protein is a Cell Growth Regulator
| Autor: | Jinglan Liu, G. Pal Kaur, Jon K. deRiel, Vikramjit K. Zhawar, Gurpreet Kaur, Raghbir S. Athwal, Raj P. Kandpal |
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| Rok vydání: | 2009 |
| Předmět: |
Simian virus 40
Biology Transfection Gene Expression Regulation Enzymologic Colony-Forming Units Assay Ribonucleases cell immortalization Cell Adhesion Humans RNA Messenger RNaseT2 Cell adhesion Gene Cell Line Transformed Cell Proliferation Cell growth Gene Expression Profiling Tumor Suppressor Proteins 6q27 Articles Cell Biology Cell Transformation Viral Molecular biology Cell biology Alternative Splicing cell senescence Cell culture Molecular Medicine Chromosomes Human Pair 6 Ectopic expression Signal transduction cell growth regulation Proto-Oncogene Proteins c-akt Immortalised cell line Signal Transduction |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| DOI: | 10.1111/j.1582-4934.2009.00749.x |
| Popis: | We have previously shown by chromosome transfer technique that chromosome 6 alters the phenotype of a variety of tumour cells and SV40 immortalized cells. We present here the phenotypic effects of the ectopic expression of RNaseT2, a highly conserved ribonuclease encoded by chromosome 6q27, in SV40 immortalized cell lines. We contrast our findings with those reported for ovarian carcinoma cell lines and an SV40 immortalized cell line transfected with RNaseT2. Although RNaseT2 expression is elevated in normal diploid fibroblasts approaching senescence (passage 64), forced expression of the gene in immortalized cells does not cause them to senesce. A significant reduction was observed in colony forming efficiency, anchorage independence and growth rate of cells transfected with RNaseT2. The levels of transcripts involved in Akt signalling pathway, cell cycle control and pathways related to cell proliferation decreased 2–10-folds in SV40 immortalized cells in response to RNaseT2 expression. Interestingly, some immortalized cells expressed alternatively spliced transcript variants instead of the full-length RNaseT2 transcript. Our results are consistent with the conclusion that RNaseT2 is a cell growth regulator and it does not induce senescence in SV40 immortalized cell lines. |
| Databáze: | OpenAIRE |
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