The inhibition of 5-Lipoxygenase (5-LO) products leukotriene B4 (LTB 4 ) and cysteinyl leukotrienes (cysLTs) modulates the inflammatory response and improves cutaneous wound healing
| Autor: | João Santana da Silva, Cristina Ribeiro de Barros Cardoso, Virmondes Rodrigues Junior, Carlos Arterio Sorgi, Javier Emílio Lazo Chica, Monique T. Fonseca, Helioswilton Sales-Campos, Francielle Rodrigues Guimarães, Lúcia Helena Faccioli, Viviani Nardini, Thiago Alvares da Costa |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Skin repair Leukotriene integumentary system biology Chemistry Leukotriene B4 Immunology Inflammation CCL2 Pharmacology BIOMARCADORES 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis Arachidonate 5-lipoxygenase biology.protein medicine Immunology and Allergy Tumor necrosis factor alpha medicine.symptom Receptor |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | To analyze the participation of the enzyme 5-lipoxygenase (5-LO) in skin repair, WT wounds were compared to those in 5-LO deficient mice (5-LO-/-), which presented faster closure and reduced inflammatory infiltrate in the skin, together with increased CD4 regulatory T cells markers in the draining lymph nodes. The 5-LO-/- wounds also had diminished TNF-α, CCL11, CCL7, CCL2, CXCL9, CCR1 and CXCR2 mRNA expression in the lesions, besides differential extracellular matrix remodeling. Furthermore, when cysteinyl leukotriene (cysLT) and leukotriene (LTB4) receptors were antagonized in WT mice, there was a remarkable reduction in TNF-α expression and faster skin healing, similarly to the findings in 5-LO-/- animals. Finally, our results suggested that 5-LO products, in special cysLT and LTB4, underline skin inflammation that follows skin injury and their neutralization may be an important strategy to improve cutaneous healing. |
| Databáze: | OpenAIRE |
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